Description

4-Methylumbelliferyl-β-D-Glucuronide is a high-purity fluorogenic substrate widely used as a diagnostic reagent in enzymatic assays. This compound is critical for the sensitive and specific detection of β-glucuronidase activity, a key biomarker in various biochemical and clinical applications. It is an essential raw material for manufacturers in the in-vitro diagnostics (IVD), pharmaceutical research, and life science research sectors, where reliable performance and consistent quality are paramount.

Application

• Clinical Diagnostics: Primary substrate for detecting β-glucuronidase activity in microbiological assays, including tests for E. coli and other coliform bacteria in water and food safety analysis.
• Pharmaceutical Research: Used in high-throughput screening (HTS) to identify and characterize inhibitors or activators of the β-glucuronidase enzyme for drug discovery.
• Molecular Biology: Essential reagent in reporter gene assays (e.g., GUS assays) for studying gene expression and regulation in plant and microbial genetics.
• Enzyme Kinetics: Serves as a standard fluorogenic substrate for kinetic studies to determine enzyme activity, specificity, and inhibition constants.
• IVD Kit Manufacturing: A key component in the formulation of diagnostic kits for urinary tract infections, bacterial identification, and other clinical microbiology tests.
• Biochemical Research: Utilized in cell-based assays and histochemistry to localize β-glucuronidase activity in tissues and cell cultures.

Basic Information

Item	Details
Product Name	4-Methylumbelliferyl-β-D-Glucuronide
CAS No.	6160-80-1
Molecular Formula	C16H16O9
Molecular Weight	352.29 g/mol
Synonyms	4-MUG; 4-Methylumbelliferyl β-D-Glucopyranosiduronic Acid; 4-Methylumbelliferyl Glucuronide; MUG; 4-MU-β-D-Glucuronide; β-Glucuronidase Substrate; 4-Methylumbelliferyl-β-D-Glucopyranosiduronic Acid; Hymecromone 7-O-β-D-glucuronide
EINECS	228-200-5

Quality Control

Our 4-Methylumbelliferyl-β-D-Glucuronide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectral identification (IR/UV), and specific activity validation in enzymatic assays. We provide full traceability and Certificates of Analysis (COA) are available for every shipment, detailing all critical quality attributes to support your regulatory and R&D requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Activity	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.