Description

Bis-Tris Propane is a high-purity, amphoteric organic compound widely recognized as an excellent biological buffer for maintaining stable pH conditions in biochemical and molecular biology applications. Its value lies in its high buffering capacity across a broad pH range (6.3 to 9.5) and minimal interference with biological processes. This makes it a critical reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries who require precise and reliable pH control.

Application

• Biochemical Research & Molecular Biology: A primary buffer component for enzyme assays, cell culture media, protein purification, electrophoresis (e.g., SDS-PAGE), and nucleic acid hybridization.
• Pharmaceutical Formulation: Used as a stabilizing agent in the development and manufacturing of biologics, vaccines, and other sensitive therapeutic products.
• Diagnostic Kits & Reagents: Provides consistent pH environments in clinical diagnostic assays, ensuring accuracy and reproducibility of results.
• Chromatography: Serves as a mobile phase modifier or buffer in various chromatographic techniques, including HPLC and affinity chromatography.
• Biopharmaceutical Production: Employed in upstream and downstream bioprocessing to maintain optimal conditions for cell growth and product stability.

Basic Information

Product Name	Bis-Tris Propane
CAS No.	64431-96-5
Molecular Formula	C8H19NO5
Molecular Weight	209.24 g/mol
Synonyms	1,3-Bis[tris(hydroxymethyl)methylamino]propane; Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane propane derivative; BTP; BIS-TRIS PROPANE; BTP Buffer; 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol propane adduct
EINECS	264-914-1

Quality Control

Our Bis-Tris Propane is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure it meets high-purity standards suitable for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect quality.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	9.5 - 10.5
Loss on Drying	≤ 0.5%
Heavy Metals (as Pb)	≤ 10 ppm
Endotoxin	< 0.1 EU/mg (for specific grades)
Solubility (in Water)	Clear, colorless solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.