Description

2-Nitrophenyl-β-D-Galactopyranoside is a chromogenic substrate specifically designed for the detection and quantification of β-galactosidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by professionals in the biotechnology, pharmaceutical, and life science research sectors for assays, molecular biology research, and diagnostic kit development.

Application

• Enzymatic Assay Substrate: A key chromogenic reagent for the colorimetric detection and kinetic study of β-galactosidase activity in vitro.
• Molecular Biology Research: Used in reporter gene assays (e.g., lacZ gene expression) to monitor gene regulation and promoter activity.
• Microbiological Testing: Employed in microbial identification systems to differentiate bacterial species based on β-galactosidase production.
• Diagnostic Kit Component: An essential raw material in the manufacturing of clinical and research ELISA kits and other diagnostic reagents.
• Biochemical Research: Used in enzyme inhibition studies and for screening potential inhibitors of β-galactosidase.
• Cell-based Assays: Applied in high-throughput screening (HTS) platforms within drug discovery to identify modulators of gene expression.

Basic Information

Product Name	2-Nitrophenyl-β-D-Galactopyranoside
CAS No.	369-07-3
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	o-Nitrophenyl-β-D-galactopyranoside; ONPG; 2-Nitrophenyl β-D-galactoside; β-D-Galactopyranoside, 2-nitrophenyl; o-Nitrophenyl-β-D-galactoside; ONP-Gal; 4-Nitrophenyl-β-D-galactopyranoside (isomer); Galactopyranoside, 2-nitrophenyl-
EINECS	206-713-5

Quality Control

Our 2-Nitrophenyl-β-D-Galactopyranoside is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by IR and NMR, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	165 - 170 °C
Specific Rotation	-60° to -65° (c=1 in water)
Water Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.