Description

Tricine CAS NO 5704-04-1 is a high-purity, zwitterionic biological buffer widely recognized for its excellent buffering capacity in the physiological pH range. This compound is critical for maintaining stable pH conditions in sensitive biochemical and molecular biology applications where minimal interference with enzymatic activity is required. It is an essential reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries, particularly for electrophoresis, cell culture media, and protein purification protocols.

Application

• Biochemical Research & Assays: A preferred buffer for enzyme kinetics studies, PCR, and other molecular biology techniques due to its low metal ion chelation and UV transparency.
• Electrophoresis: Commonly used in SDS-PAGE and other gel electrophoresis systems as an alternative to Tris-glycine buffers, especially for separating low molecular weight proteins.
• Cell Culture & Media Preparation: Employed to stabilize pH in mammalian and bacterial cell culture media and related solutions.
• Protein Purification & Crystallography: Provides a stable, non-interfering environment for chromatography (e.g., ion-exchange, gel filtration) and protein crystallization screens.
• Diagnostic Reagent Formulation: Used as a buffering agent in the production of clinical diagnostic kits and reagents to ensure consistent performance.
• Pharmaceutical Development: Serves as an excipient or buffer component in the formulation of biopharmaceuticals and vaccines.

Basic Information

Product Name	Tricine
CAS No.	5704-04-1
Molecular Formula	C6H13NO5
Molecular Weight	179.17 g/mol
Synonyms	N-[Tris(hydroxymethyl)methyl]glycine; Tris-Glycine; N-(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine; Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-; Tris(hydroxymethyl)methylglycine; TRICINE buffer; Biological buffer Good's buffer
EINECS	227-193-6

Quality Control

Our Tricine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and production. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and stringent limits for heavy metals and endotoxins. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We can supply grades compliant with relevant pharmacopeial standards (e.g., USP, EP) upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed after each use to prevent absorption of atmospheric moisture, which can affect quality and weight.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference standard
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	3.8 - 4.2
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm
Endotoxin (where applicable)	< 0.1 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.