Description

4-Methylumbelliferyl-β-D-Glucopyranoside is a high-purity fluorogenic substrate specifically designed for enzymatic assays. This compound is critical for the sensitive and quantitative detection of β-glucosidase activity, a key enzyme in numerous biochemical and diagnostic applications. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries, where precise enzyme measurement is paramount.

Application

• Enzyme Activity Assays: Primary substrate for the fluorometric determination of β-glucosidase activity in research and clinical diagnostics.
• Diagnostic Kits: Key component in diagnostic kits for genetic disorders like Gaucher disease, where β-glucosidase deficiency is measured.
• Microbiology & Cell Biology: Used as a reporter substrate to study gene expression and enzyme localization in microbial and mammalian cell systems.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential inhibitors or activators of β-glucosidase for drug discovery.
• Food & Environmental Analysis: Applied in assays to detect microbial contamination or specific enzymatic activities in food safety and environmental monitoring.
• Biochemical Research: Fundamental tool for kinetic studies, enzyme characterization, and mechanistic investigations of glycoside hydrolases.

Basic Information

Product Name	4-Methylumbelliferyl-β-D-Glucopyranoside
CAS No.	18997-57-4
Molecular Formula	C16H18O9
Molecular Weight	354.31 g/mol
Synonyms	4-MU-β-D-Glucoside; 4-Methylumbelliferyl β-D-Glucoside; 4-MUG; 4-Methylumbelliferyl-β-D-glucopyranoside; β-Glucosidase Substrate; 4-MU-Glc; 7-Hydroxy-4-methylcoumarin β-D-glucopyranoside; 4-Methyl-7-β-D-glucopyranosyloxycoumarin
EINECS	242-758-5

Quality Control

Our 4-Methylumbelliferyl-β-D-Glucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectrophotometric verification. A Certificate of Analysis (COA) detailing identity, purity, and specific performance data is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.