Description

Bis-Tris Hydrochloride CAS NO 124763-51-5 is a high-purity organic buffer salt widely used to maintain stable pH conditions in sensitive biochemical and molecular biology applications. Its primary value lies in its excellent buffering capacity in the physiological pH range (pKa ~6.5 at 25°C) and its low metal ion binding, which helps prevent enzyme inhibition. This makes it an essential reagent for researchers and manufacturers in the biopharmaceutical, diagnostic, and life science industries who require precise and non-interfering buffer systems.

Application

• Biochemical & Molecular Biology Buffers: A key component in electrophoresis (e.g., Bis-Tris gels for protein separation), nucleic acid purification, and enzyme reaction mixtures.
• Cell Culture & Media Preparation: Used to maintain stable pH in cell culture media and related solutions for mammalian and bacterial cell growth.
• Pharmaceutical Formulation: Serves as a buffering agent in the development and manufacturing of injectable drugs, vaccines, and diagnostic reagents.
• Protein Crystallography & Stabilization: Provides a stable pH environment for protein structure analysis and long-term protein storage solutions.
• Chromatography: Utilized as a mobile phase buffer in various chromatographic techniques, including HPLC and FPLC, for biomolecule separation.
• Diagnostic Kits: Incorporated as a buffer in clinical diagnostic assays and kits to ensure consistent and accurate test performance.

Basic Information

Product Name	Bis-Tris Hydrochloride
CAS No.	124763-51-5
Molecular Formula	C8H19NO5 • HCl
Molecular Weight	245.70 g/mol
Synonyms	2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol hydrochloride; Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane hydrochloride; BIS-TRIS HCl; BIS-TRIS•HCl; BTH; 1,3-Bis[tris(hydroxymethyl)methylamino]propane hydrochloride (common misnomer); Bis-Tris buffer hydrochloride
EINECS	Contact for details

Quality Control

Our Bis-Tris Hydrochloride is manufactured under a strict quality management system. Each batch undergoes rigorous analytical testing to ensure it meets high-purity standards suitable for research and pharmaceutical applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed after opening to prevent moisture absorption, which can affect product performance and stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	4.5 - 5.5
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm
Chloride Content (Titration)	13.5 - 14.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.