Description

Bis-Tris CAS NO 6976-37-0 is a high-purity, zwitterionic organic compound widely recognized as an excellent biological buffer. Its primary value lies in its effective buffering capacity in the physiological pH range (pKa ~6.5 at 25°C) with minimal interference in biochemical systems. This product is essential for researchers and manufacturers in the life sciences, biotechnology, and pharmaceutical industries who require precise pH control for sensitive applications such as protein purification, cell culture, and diagnostic assay formulation.

Application

• Biochemical & Molecular Biology Research: A preferred buffer component in electrophoresis (e.g., Bis-Tris gels for protein separation), chromatography, and enzyme assays due to its low UV absorbance and metal chelating properties.
• Pharmaceutical Formulation: Used as a buffering agent in the development and manufacturing of injectable drugs, vaccines, and diagnostic reagents to maintain stable pH.
• Cell Culture Media: Employed to stabilize the pH of cell culture and fermentation media, supporting optimal growth conditions for mammalian and bacterial cells.
• Protein Purification & Crystallization: Provides a stable, non-interfering environment for purifying proteins via column chromatography and for growing protein crystals in structural biology studies.
• Diagnostic Kits: Acts as a key buffer in the formulation of clinical diagnostic kits, including ELISA and other immunoassays, ensuring consistent and reliable performance.

Basic Information

Product Name	Bis-Tris
CAS No.	6976-37-0
Molecular Formula	C8H19NO5
Molecular Weight	209.24 g/mol
Synonyms	2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol; Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane; BIS-TRIS; Bistris; BIS-TRIS Buffer; BTP; 1,3-Bis[tris(hydroxymethyl)methylamino]propane (common misnomer for related compound); Bicine precursor
EINECS	230-237-4

Quality Control

Our Bis-Tris is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure it meets high-purity standards suitable for research and pharmaceutical applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with every shipment. We can supply grades compliant with relevant internal specifications for biopharmaceutical use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the original container is tightly sealed after each use to prevent absorption of atmospheric moisture, which can affect purity and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	9.5 - 10.5
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm
UV Absorbance (1M, 260 nm)	≤ 0.05 AU
UV Absorbance (1M, 280 nm)	≤ 0.03 AU

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.