Description

6-[2-Carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-1,2,10,11-tetrahydro-2,2,10,10-tetramethyl-4,8-bis(sulfomethyl)pyrano[3,2-g:5,6-g']diquinolin-13-ium inner salt is a highly specialized, functionalized fluorescent dye intermediate. This compound is engineered for advanced bioconjugation applications, featuring a reactive N-hydroxysuccinimide (NHS) ester group that enables efficient covalent bonding to primary amines. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies developing next-generation fluorescent probes, sensors, and labeling reagents.

Application

• Fluorescent Probe Synthesis: Serves as a key precursor for creating reactive, water-soluble fluorescent dyes with enhanced brightness and photostability.
• Bioconjugation & Labeling: The NHS ester functionality allows for the covalent labeling of proteins, antibodies, peptides, and oligonucleotides for fluorescence-based assays (e.g., ELISA, flow cytometry).
• Diagnostic Reagent Manufacturing: Used in the production of fluorescent tags and detection reagents for in vitro diagnostics (IVD) and clinical chemistry.
• Biomedical Imaging: Development of contrast agents and molecular imaging probes for cellular and in vivo fluorescence imaging studies.
• Sensor Development: Integration into chemical and biological sensors where a fluorescent signal change indicates the presence of a specific analyte.
• Material Science: Functionalization of nanoparticles and polymers to create fluorescently tagged materials for tracking and analysis.

Basic Information

Product Name	6-[2-Carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-1,2,10,11-tetrahydro-2,2,10,10-tetramethyl-4,8-bis(sulfomethyl)pyrano[3,2-g:5,6-g']diquinolin-13-ium inner salt
CAS No.	878549-44-1
Molecular Formula	C41H37N3O11S2
Molecular Weight	811.88 g/mol
Synonyms	Pyranine NHS ester; HPTS NHS ester; 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt NHS ester derivative; Hydroxypyrene trisulfonic acid NHS ester; Fluorescent NHS ester dye; Reactive pyranine derivative; 6-[2-Carboxy-4(5)-(N-succinimidyloxycarbonyl)phenyl]-1,2,10,11-tetrahydro-2,2,10,10-tetramethyl-4,8-bis(sulfomethyl)pyrano[3,2-g:5,6-g']diquinolin-13-ium
EINECS	Contact for details

Quality Control

Every batch of this high-purity intermediate is subjected to rigorous analytical testing to ensure consistency and performance in sensitive conjugation reactions. Our quality assurance protocol includes HPLC for purity and identity confirmation, NMR structural verification, and functional group analysis. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment to support your quality management systems.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the NHS ester group is susceptible to hydrolysis. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water
Functional Group Assay (NHS ester)	≥ 90% active ester

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.