Description

1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-6,8-disulfo-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-6,8-disulfo-, inner salt, potassium salt (1:3) is a highly functionalized cyanine dye derivative, engineered for advanced applications requiring specific molecular conjugation. This compound matters for its reactive succinimidyl ester group, which enables stable covalent bonding to amines, making it an essential tool for labeling and detection. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and material science sectors for developing sensitive probes and functional materials.

Application

• Fluorescent Labeling Reagent: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for fluorescence microscopy, flow cytometry, and immunoassays.
• Bioconjugation & Probe Synthesis: Key intermediate in the synthesis of near-infrared (NIR) fluorescent probes and molecular beacons for in vivo imaging and biodetection.
• Diagnostic Assay Development: Used in the manufacture of diagnostic kits and biosensors that require high-sensitivity, stable fluorescent signals.
• Material Science Functionalization: Surface modification of nanoparticles, polymers, and other materials to impart specific optical or binding properties.
• Photodynamic Therapy (PDT) Research: Investigated as a potential photosensitizer precursor due to its extended conjugated system and water-solubilizing sulfonate groups.
• DNA/RNA Staining & Detection: Development of specialized stains for gel electrophoresis and nucleic acid quantification assays.

Basic Information

Item	Details
Product Name	1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-6,8-disulfo-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-6,8-disulfo-, inner salt, potassium salt (1:3)
CAS No.	2419286-92-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cyanine5.5 NHS ester; Sulfo-Cy5.5 NHS ester; Cy5.5 succinimidyl ester, sulfonated; 1,1,3,3,1',1',3',3'-Octamethyl-6,6',8,8'-tetrasulfo-indodicarbocyanine NHS ester, potassium salt; N-Hydroxysuccinimide ester of Cy5.5 dye; Reactive Cy5.5 dye; Water-soluble Cy5.5 NHS ester; Pentamethine cyanine dye NHS ester, sulfonated
EINECS	Contact for details

Quality Control

Our 1H-Benz[e]indolium dye derivative is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometric analysis (absorbance/fluorescence), and functional group verification (e.g., NHS ester activity). A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation and hydrolysis of the reactive ester group.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Max (λmax)	Contact for details
Emission Max (λem)	Contact for details
Extinction Coefficient (ε)	Contact for details
Moisture Content (KF)	≤ 5.0%
Functional Activity	Conforms to labeling efficiency test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.