Description

4-Nitrophenyl O-β-D-Galactopyranosyl-(1→4)-O-2-(acetylaMino)-2-deoxy-β-D-glucopyranosyl-(1→3)-O-β-D-galactopyranosyl-(1→4)-2-(acetylaMino)-2-deoxy-β-D-glucopyranoside is a specialized synthetic oligosaccharide derivative, serving as a critical chromogenic substrate for enzyme activity studies. Its value lies in providing a precise and measurable signal for the detection and quantification of specific glycosidase enzymes. This compound is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors who require high-purity biochemical tools.

Application

• Enzymatic Substrate for Glycosidases: Primarily used as a chromogenic substrate for the detection and kinetic analysis of specific β-galactosidases and related enzymes.
• Biochemical Research: A key reagent in studying enzyme mechanisms, inhibition, and substrate specificity within complex carbohydrate pathways.
• Diagnostic Kit Development: Employed in the formulation of diagnostic assays and kits for clinical and research purposes to detect enzymatic activity.
• Pharmaceutical Quality Control: Used in purity and activity testing of enzyme-based biopharmaceuticals during manufacturing and release.
• Microbiology Studies: Applied in assays to identify and characterize bacterial strains based on their specific glycosidase profiles.

Basic Information

Product Name	4-Nitrophenyl O-β-D-Galactopyranosyl-(1→4)-O-2-(acetylaMino)-2-deoxy-β-D-glucopyranosyl-(1→3)-O-β-D-galactopyranosyl-(1→4)-2-(acetylaMino)-2-deoxy-β-D-glucopyranoside
CAS No.	253136-44-6
Molecular Formula	C40H61N3O27
Molecular Weight	1015.91 g/mol
Synonyms	4-Nitrophenyl β-D-galactopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranosyl-(1→3)-β-D-galactopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranoside; p-Nitrophenyl-β-D-Gal-(1→4)-β-D-GlcNAc-(1→3)-β-D-Gal-(1→4)-β-D-GlcNAc; Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ-pNP; Lacto-N-neotetraose p-nitrophenyl derivative; pNP-LNnT; Chromogenic LNnT substrate; 4-Nitrophenyl lacto-N-neotetraoside
EINECS	Contact for details

Quality Control

Every batch of this biochemical reagent is produced under strict quality management and undergoes comprehensive analytical testing to ensure identity, purity, and performance. Specifications are aligned with research-grade standards, and a detailed Certificate of Analysis (COA) documenting purity (by HPLC), identification (IR/NMR), and other critical parameters is provided with each shipment to support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.