Description

Phosphoramidous acid, bis(1-methylethyl)-, 2-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-3-(methylamino)-2-[(methylamino)carbonyl]-3-oxopropyl 2-cyanoethyl ester (9CI) is a sophisticated, high-purity phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. Its value lies in its role as a key reagent for the controlled, stepwise assembly of DNA and RNA sequences, ensuring high coupling efficiency and fidelity. This compound is essential for researchers and manufacturers in the fields of therapeutics, diagnostics, and genetic research, where precision and reliability are paramount.

Application

• Solid-Phase Oligonucleotide Synthesis: As a phosphoramidite monomer for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Production: Manufacture of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical applications.
• Diagnostic Probe Development: Synthesis of labeled probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Research-Grade Oligo Synthesis: Production of primers, linkers, and gene fragments for academic and industrial R&D.
• Synthesis of Modified Oligonucleotides: Enabling the incorporation of specific modifications for enhanced stability or functionality.
• Scale-Up cGMP Manufacturing: Suitable for use in controlled environments for the production of clinical-grade nucleic acid APIs.

Basic Information

Product Name	Phosphoramidous acid, bis(1-methylethyl)-, 2-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-3-(methylamino)-2-[(methylamino)carbonyl]-3-oxopropyl 2-cyanoethyl ester (9CI)
CAS No.	202284-84-2
Molecular Formula	C43H52N3O8P
Molecular Weight	769.87 g/mol
Synonyms	5'-O-DMT-N2-methyl-2'-O-[(N-methyl)carbamoyl]guanosine 3'-(2-cyanoethyl diisopropylphosphoramidite); N2-Methyl-2'-O-(N-methylcarbamoyl)guanosine 5'-O-(4,4'-dimethoxytrityl)-3'-O-(2-cyanoethyl diisopropylphosphoramidite); 2'-O-(N-Methylcarbamoyl)-N2-methylguanosine 5'-DMT 3'-CE phosphoramidite; G(mcm) Phosphoramidite; RNA Phosphoramidite (mcmG); 2'-O-Methylcarbamoyl-N2-methyl-Guanosine Phosphoramidite; Contact for additional synonyms.
EINECS	Contact for details

Quality Control

This high-value phosphoramidite is produced under strict quality control protocols to ensure batch-to-batch consistency and optimal performance in synthesis. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and 31P NMR to confirm identity, purity, and reactivity. Certificates of Analysis (COA) detailing all specifications are provided and can be tailored to meet specific customer or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
31P NMR Purity	Single major peak conforming

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.