Description

Lithocholyl-n-Hydroxysuccinimide is a high-purity, activated ester derivative of lithocholic acid, specifically designed for bioconjugation and peptide synthesis. This compound matters as a critical reagent for the efficient and site-specific labeling of biomolecules, enabling advanced research in diagnostics and drug development. It is primarily needed by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research laboratories focused on bile acid metabolism, probe development, and bioconjugate chemistry.

Application

• Bioconjugation Reagent: Used for the covalent coupling of lithocholic acid or its derivatives to proteins, peptides, or other amine-containing molecules for probe and tracer development.
• Peptide Synthesis Intermediate: Serves as a key activated building block in solid-phase peptide synthesis (SPPS) for incorporating bile acid motifs.
• Diagnostic Assay Development: Essential for creating enzyme-linked immunosorbent assay (ELISA) components, chemiluminescent probes, and other diagnostic conjugates.
• Metabolic Pathway Research: Employed in the study of bile acid receptors (e.g., TGR5, FXR) and their signaling pathways by creating labeled ligands.
• Drug Delivery System Research: Utilized to synthesize prodrugs or targeted drug conjugates that exploit bile acid transport mechanisms.
• Biomaterial Functionalization: Applied to modify surfaces or nanoparticles with bile acid moieties to enhance biocompatibility or cellular targeting.

Basic Information

Product Name	Lithocholyl-n-Hydroxysuccinimide
CAS No.	104211-94-1
Molecular Formula	C28H43NO5
Molecular Weight	473.65 g/mol
Synonyms	Lithocholic Acid N-Hydroxysuccinimide Ester; 3α-Hydroxy-5β-cholan-24-oic Acid N-Succinimidyl Ester; Lithocholyl-OSu; Lithocholyl-NHS; N-Hydroxysuccinimide Lithocholate; 5β-Cholan-24-oic Acid, 3-hydroxy-, 1,1-dimethylethyl Ester; Lithocholyl Succinimidyl Ester; NSC 334906
EINECS	Contact for details

Quality Control

Our Lithocholyl-n-Hydroxysuccinimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed batch-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.