Description

1-[(5E)-3-O-[(Bis-di-isopropyl amino)(2-cyanoethoxy) phosphino]-5,6-dideoxy-6-(diethoxyphosphinyl)-2-O-(2-methoxyethyl)-β-D-ribo-hex-5-enofuranosyl]-5-methyluracil is a sophisticated phosphoramidite monomer, a critical building block in the automated synthesis of oligonucleotides. Its value lies in enabling the precise, high-yield incorporation of modified nucleosides, which is essential for advanced research and therapeutic development. This compound is primarily needed by biotechnology and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent for the automated synthesis of modified DNA/RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and aptamer drugs.
• Research & Development: Synthesis of custom oligonucleotide probes and primers with specific modifications for diagnostic and genomic research.
• Synthesis of Nucleoside Analogs: Used in the chemical synthesis of complex nucleoside derivatives for structure-activity relationship (SAR) studies.
• Bioconjugation: Facilitating the site-specific labeling or functionalization of oligonucleotides for diagnostic and targeting applications.

Basic Information

Item	Detail
Product Name	1-[(5E)-3-O-[(Bis-di-isopropyl amino)(2-cyanoethoxy) phosphino]-5,6-dideoxy-6-(diethoxyphosphinyl)-2-O-(2-methoxyethyl)-β-D-ribo-hex-5-enofuranosyl]-5-methyluracil
CAS No.	1345562-47-1
Molecular Formula	C31H53N4O11P2
Molecular Weight	710.72 g/mol
Synonyms	5-Methyluridine C-6 phosphonate phosphoramidite; 2'-O-(2-Methoxyethyl)-5-methyluridine 5'-(6-diethylphosphonate) 3'-(2-cyanoethyl N,N-diisopropyl)phosphoramidite; 5-Me-Urd(MOE) Phosphoramidite; 2'-MOE-5-methyluridine C-6 phosphonate amidite; 5-Methyl-2'-O-(2-methoxyethyl)uridine-3'-(2-cyanoethyl diisopropylphosphoramidite)-6-diethylphosphonate; Modified Nucleoside Phosphoramidite
EINECS	Contact for details

Quality Control

Our production of this high-value phosphoramidite adheres to strict quality protocols to ensure batch-to-batch consistency and optimal coupling efficiency. Every lot is characterized using advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and performance data is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will degrade product quality and performance.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.