Description

2-Hydroxyestrone 2-methyl-13C,d3-ether, 2-Methoxy-13C,d3-estrone, 3-Hydroxy-2-methoxy-13C,d3-1,3,5(10)-estratrien-17-one, 2,3-Dihydroxy-1,3,5(10)-estratrien-17-one-2-methyl-13C,d3-ether is a high-purity, stable isotope-labeled analog of endogenous estrogen metabolites, specifically designed for use as an internal standard in advanced analytical techniques. This compound is critical for ensuring quantitative accuracy and method validation in complex biological matrices, eliminating variability and enhancing data reliability. It is an essential tool for research and diagnostic laboratories in endocrinology, clinical research, and pharmaceutical development, where precise measurement of hormone metabolites is paramount.

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for the analysis of endogenous estrogen metabolites in serum, plasma, and urine.
• Clinical Research & Diagnostics: Enables precise profiling of estrogen metabolism pathways for studies related to hormone-dependent cancers, metabolic disorders, and endocrine function.
• Pharmaceutical R&D: Used in drug metabolism and pharmacokinetic (DMPK) studies to track the biotransformation of estrogen-based therapeutics and novel drug candidates.
• Biomarker Discovery & Validation: Supports the development and validation of robust clinical assays for specific hydroxyestrone isomers as potential disease biomarkers.
• Forensic & Anti-Doping Analysis: Serves as a critical reference material in confirmatory testing for endogenous steroid profiles in forensic toxicology and sports anti-doping programs.
• Method Development & Transfer: Facilitates the development, optimization, and transfer of highly sensitive and specific analytical methods between laboratories.
• Quality Control in Reference Material Production: Used in the calibration and quality control of certified reference materials (CRMs) for clinical chemistry.

Basic Information

Item	Details
Product Name	2-Hydroxyestrone 2-methyl-13C,d3-ether, 2-Methoxy-13C,d3-estrone, 3-Hydroxy-2-methoxy-13C,d3-1,3,5(10)-estratrien-17-one, 2,3-Dihydroxy-1,3,5(10)-estratrien-17-one-2-methyl-13C,d3-ether
CAS No.	1217460-84-8
Molecular Formula	C19H2213CD3O3 (Exact isotopic composition may vary)
Molecular Weight	~305.4 g/mol (Contact for exact isotopic mass)
Synonyms	2-Methoxy-13C,d3-estrone; 2-Hydroxyestrone 2-methyl-13C,d3-ether; [13C,d3]-2-Methoxyestrone; 3-Hydroxy-2-methoxy-13C,d3-estra-1,3,5(10)-trien-17-one; 2,3-Dihydroxyestra-1,3,5(10)-trien-17-one 2-methyl-13C,d3-ether; 13C and Deuterium Labeled 2-Methoxyestrone; Isotopically Labeled Estrogen Metabolite; Stable Isotope-Labeled Internal Standard for Estrone Analysis.
EINECS	Contact for details

Quality Control

Every batch of our stable isotope-labeled compounds is produced and analyzed under strict quality control protocols to ensure isotopic purity, chemical purity, and stability. Our rigorous testing includes advanced spectroscopic and chromatographic methods to confirm identity and quantify isotopic enrichment. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing batch-specific data for purity (HPLC), isotopic purity (MS), and other critical parameters. We support compliance with GLP and ISO 17025 standards for analytical testing.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Under these conditions, the product is stable for at least 24 months from the date of receipt. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation. The compound is intended for research use only in a laboratory setting.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 99 atom % 13C; ≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.