Description

Leishman's Stain is a critical histological and cytological dye mixture used for the differential staining of blood cells and tissue samples. Its primary value lies in its ability to clearly distinguish between different types of white blood cells and to identify parasites like Leishmania and malaria within blood smears. This product is essential for professionals in clinical diagnostics, hematology research, and veterinary pathology who require reliable and consistent staining for accurate microscopic analysis. We supply high-purity Leishman's Stain to ensure reproducible and diagnostically significant results.

Application

• Hematological Staining: Primary application for differential staining of peripheral blood smears to identify and classify leukocytes (neutrophils, lymphocytes, monocytes, eosinophils, basophils).
• Parasitology: Detection and identification of blood-borne parasites, including Plasmodium species (malaria) and Leishmania species in clinical specimens.
• Bone Marrow Examination: Used in the analysis of bone marrow aspirates to assess cell morphology and diagnose hematological disorders.
• Veterinary Diagnostics: Employed in veterinary clinics and laboratories for blood film analysis in animals.
• Cytology: Staining of other bodily fluids and fine-needle aspiration samples for cellular examination.
• Research & Development: Essential reagent in life science research, particularly in immunology, hematology, and infectious disease studies.
• Quality Control in Manufacturing: Used by manufacturers of diagnostic kits and stains for batch consistency testing.

Basic Information

Product Name	Leishman's Stain
CAS No.	12627-53-1
Molecular Formula	Mixture (Primarily C15H16ClN3S and C20H20ClN3)
Molecular Weight	Mixture
Synonyms	Leishman Stain; Leishman's Blood Stain; Romanowsky Stain (Modified); Leishman's Eosin-Methylene Blue; CI 52030 (component); Methylene Blue Eosinate; Azure B-Eosin Y complex
EINECS	Contact for details

Quality Control

Our Leishman's Stain is manufactured and tested to ensure consistent staining performance and batch-to-batch reproducibility. Each lot undergoes rigorous quality testing, including assessment of staining intensity, pH, solubility, and absence of precipitates. A Certificate of Analysis (COA) detailing these parameters is available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at a controlled room temperature between 15-25°C (59-77°F). Keep the container dry and in a well-ventilated place. The product is light-sensitive and should be stored away from direct light exposure to prevent degradation.

Specification

Item	Specification
Appearance	Green powder or crystalline solid
Identification (IR)	Conforms to structure
Solubility (in Methanol)	Completely soluble, clear solution
Staining Performance	Produces characteristic differentiation of blood cell nuclei and cytoplasm
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.