Description

4-Hydroxyestradiol 4-methyl-13C,d3 ether, also known as 4-Methoxy-13C,d3-estradiol, is a high-purity, stable isotope-labeled derivative of estradiol, a key endogenous estrogen. This compound is specifically engineered for use as an internal standard in quantitative mass spectrometry, providing unmatched accuracy and reliability for trace-level analysis. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and environmental monitoring sectors who require precise quantification of estradiol and its metabolites.

Application

• Internal Standard for LC-MS/MS: Primary application for the precise quantification of estradiol and its hydroxylated metabolites in complex biological matrices like serum, plasma, and urine.
• Pharmaceutical R&D: Used in pharmacokinetic and pharmacodynamic studies to track drug metabolism and hormone disposition.
• Clinical Research & Diagnostics: Enables accurate measurement of hormonal levels for studies on endocrinology, oncology (e.g., breast cancer), and reproductive health.
• Environmental Analysis: Facilitates the detection and quantification of estrogenic compounds in water and soil samples.
• Metabolomics & Biomarker Discovery: Serves as a critical reference compound in high-throughput profiling of steroid hormone pathways.
• Method Development & Validation: Essential for developing, calibrating, and validating robust analytical methods in compliance with FDA, EMA, and other regulatory guidelines.

Basic Information

Product Name	4-Hydroxyestradiol 4-methyl-13C,d3 ether
CAS No.	1217442-62-0
Molecular Formula	C19H2413CD3O3 (Exact composition depends on isotopic labeling sites)
Molecular Weight	Contact for details
Synonyms	4-Methoxy-13C,d3-estradiol; 1,3,5(10)-Estratriene-2,3,17-triol 4-methyl-13C,d3 ether; 3,4,17β-Trihydroxy-1,3,5(10)-estratriene 4-methyl-13C,d3 ether; 3,17β-Dihydroxy-4-methoxy-13C,d3-1,3,5(10)-estratriene; [13C,d3]-4-Methoxyestradiol; 4-O-Methyl-13C,d3-estradiol; Stable Isotope-Labeled 4-Hydroxyestradiol Methyl Ether; Deuterated and 13C-Labeled Estradiol Metabolite
EINECS	Contact for details

Quality Control

Every batch of our stable isotope-labeled 4-Hydroxyestradiol 4-methyl-13C,d3 ether is manufactured and tested under strict quality systems. We guarantee high chemical and isotopic purity as verified by advanced analytical techniques including NMR, HPLC, and high-resolution mass spectrometry. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, isotopic enrichment, and analytical data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms
Purity (HPLC)	≥ 98%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.