Description

(2R,3R,4R,5R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-((4-nitrobenzyloxy)methoxy)tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite is a sophisticated phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its value lies in its precisely defined stereochemistry and protecting group strategy, which ensures the high-fidelity incorporation of nucleosides into complex DNA and RNA sequences. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, high-purity reagents for antisense therapy development, PCR primers, probes, and other advanced nucleic acid applications.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated solid-phase synthesis of DNA and RNA strands.
• Antisense & siRNA Therapeutics: Production of research-grade and therapeutic oligonucleotides for gene silencing and targeted drug development.
• Diagnostic Probe Manufacture: Synthesis of labeled probes for PCR, microarrays, fluorescence in situ hybridization (FISH), and other molecular diagnostic tools.
• Primer Production: Creation of high-purity primers for sequencing, cloning, and amplification techniques.
• Research & Development: Use in academic and industrial R&D for studying nucleic acid chemistry, structure, and function.
• Synthesis of Modified Oligonucleotides: Enabling the introduction of specific, protected nucleoside analogs into synthetic oligonucleotides for specialized applications.

Basic Information

Product Name	(2R,3R,4R,5R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-((4-nitrobenzyloxy)methoxy)tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite
CAS No.	175473-95-7
Molecular Formula	C48H53N4O12P
Molecular Weight	909.93 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-[(4-nitrobenzyloxy)methyl]-N3-benzoyl-3'-O-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite]uridine; 2'-O-(4-Nitrobenzyloxymethyl)-5'-O-(4,4'-dimethoxytrityl)-N3-benzoyluridine 3'-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite]; 2'-O-NBM-5'-O-DMT-N3-Bz-uridine 3'-CE phosphoramidite; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(4-nitrophenyl)methoxy]methyl]-N3-benzoyl-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; NBom-U Phosphoramidite (N3-Bz protected); 2'-O-(4-Nitrobenzyloxymethyl)uridine phosphoramidite
EINECS	Contact for details

Quality Control

Our (2R,3R,4R,5R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-((4-nitrobenzyloxy)methoxy)tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite is manufactured under strict quality systems suitable for research and pharmaceutical development. Each batch undergoes rigorous analytical testing, including HPLC purity analysis and 31P & 1H NMR spectroscopy, to ensure identity, purity, and performance in oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C under an inert atmosphere (argon or nitrogen). This compound is easily oxidized and moisture-sensitive; allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 97.0% (Area Percent)
Water Content (KF)	≤ 0.5%
Assay (NMR)	≥ 95.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.