Description

Cytidine, N-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-5-methyl-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite] (9CI) is a highly specialized, protected phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity during the production of modified DNA and RNA strands. It is an essential raw material for pharmaceutical R&D, diagnostic development, and advanced life science research, particularly in the fields of antisense therapeutics, siRNA, and aptamers.

Application

• Oligonucleotide Synthesis: Primary building block for the automated, solid-phase synthesis of DNA and RNA sequences containing 5-methylcytidine modifications.
• Therapeutic Oligonucleotide Manufacturing: Key raw material in the production of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers for clinical development and commercial manufacturing.
• Diagnostic Probe Development: Used to synthesize labeled or modified probes for PCR, microarrays, fluorescence in situ hybridization (FISH), and other molecular diagnostic assays.
• Research-Grade Reagent: Employed in academic and industrial laboratories for custom oligonucleotide synthesis to support basic research in genetics, molecular biology, and biochemistry.
• Synthesis of Modified Nucleosides: Serves as a protected intermediate for the further chemical elaboration of nucleoside analogs with potential antiviral or anticancer activity.

Basic Information

Item	Detail
Product Name	Cytidine, N-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-5-methyl-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite] (9CI)
CAS No.	160107-14-2
Molecular Formula	C55H69N4O9PSi
Molecular Weight	989.21 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-5-methyl-N-benzoyl-cytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5-Me-CE Phosphoramidite (DMT, 2'-O-TBDMS, N-Bz); 5-Methylcytidine Phosphoramidite (protected); 2'-O-TBDMS-5-methyl-N-benzoyl-cytidine-3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 5-Methyl-dC Phosphoramidite; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-5-methylcytidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

This high-purity phosphoramidite is manufactured under strict quality control in a dedicated synthesis environment to prevent cross-contamination. Each batch is characterized by NMR (1H, 31P) and HPLC to ensure structural identity and chemical purity. We provide comprehensive Certificates of Analysis (COA) with detailed specifications for critical parameters including assay, moisture content, and purity relative to critical impurities. Our quality system supports the needs of GMP-compliant therapeutic oligonucleotide production.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (NMR)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.