Description

Maleimide-PEG2-NHS Ester is a heterobifunctional crosslinker featuring a maleimide group for thiol conjugation and an NHS ester for primary amine coupling. This compound is essential for creating stable, site-specific bioconjugates with controlled spacing and reduced steric hindrance. It is a critical reagent for researchers and manufacturers in biopharmaceutical development, antibody-drug conjugate (ADC) production, and advanced diagnostic assay formulation.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic payloads to cysteine residues on monoclonal antibodies.
• Protein and Peptide Labeling: Fluorescent or functional tag attachment to proteins, peptides, or other biomolecules containing free thiols or amines.
• Surface Functionalization: Modification of amine-coated surfaces (e.g., biosensor chips, nanoparticles) with thiol-containing ligands or proteins.
• PEGylation Reagent: Introduction of a short, hydrophilic polyethylene glycol (PEG) spacer to improve solubility and pharmacokinetics of therapeutic molecules.
• Diagnostic Reagent Synthesis: Construction of enzyme-antibody or hapten-protein conjugates for ELISA and other immunoassays.
• Hydrogel and Biomaterial Crosslinking: Creating covalently crosslinked networks for tissue engineering and drug delivery systems.

Basic Information

Product Name	Maleimide-PEG2-NHS Ester
CAS No.	955094-26-5
Molecular Formula	C17H22N2O8
Molecular Weight	382.37 g/mol
Synonyms	N-(2-{2-[2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy]ethoxy}ethyl)-2,5-dioxo-1-pyrrolidinesulfonamide; Maleimide-PEG2-NHS ester; NHS-PEG2-Maleimide; SM(PEG)2; 1-[2-[2-[2-[[(2,5-Dioxopyrrolidin-1-yl)oxy]-2-oxoethoxy]ethoxy]ethyl]-1H-pyrrole-2,5-dione; Succinimidyl ester of Maleimide-PEG2-acid; MAL-PEG2-NHS; Bifunctional PEG linker (NHS/Maleimide)
EINECS	Contact for details

Quality Control

Our Maleimide-PEG2-NHS Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high reactivity. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional group integrity. Certificates of Analysis (COA) with detailed specifications are provided to support your research and cGMP manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.