Description

Adenosine 5'-(Trihydrogen Diphosphate), P'-5-Ester With 4-Nitrophenyl β-D-Ribofuranoside is a specialized nucleotide derivative, commonly utilized as a chromogenic substrate in enzymatic assays. This compound is critical for researchers and manufacturers requiring precise detection and quantification of enzymatic activity, particularly for phosphatases and related enzymes. It serves as an essential tool in biochemical research, pharmaceutical development, and diagnostic kit formulation, where reliable and sensitive substrate performance is paramount.

Application

• Enzymatic Assay Substrate: Primary use as a chromogenic substrate for alkaline phosphatase (ALP) and other phosphatase enzymes in research and diagnostic applications.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibition assays to understand catalytic mechanisms.
• Diagnostic Kit Manufacturing: A key component in the production of clinical diagnostic kits for measuring enzyme levels in biological samples.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential therapeutic enzyme inhibitors.
• Life Science Reagents: Formulated into ready-to-use reagent solutions for laboratory analysis in academic, clinical, and industrial settings.
• Quality Control Testing: Used in biomanufacturing QC to monitor the activity of enzyme products or processes.

Basic Information

Product Name	Adenosine 5'-(Trihydrogen Diphosphate), P'-5-Ester With 4-Nitrophenyl β-D-Ribofuranoside
CAS No.	939028-75-8
Molecular Formula	C20H26N6O16P2
Molecular Weight	692.40 g/mol
Synonyms	4-Nitrophenyl Adenosine-5'-Monophosphate; p-Nitrophenyl Adenosine Monophosphate; pNPP-AMP; PNPP-AMP; 4-Nitrophenyl-AMP; Adenosine 5'-monophosphate 4-nitrophenyl ester; 4-Nitrophenyl adenosine-5'-phosphate; 5'-AMP 4-nitrophenyl ester
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Each batch is characterized and tested to ensure identity, purity, and functional performance as a reliable enzyme substrate. Comprehensive analysis includes HPLC for purity, spectroscopic methods for identification, and functional testing in standardized enzyme assays. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment to support your research and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed under anhydrous conditions after each use to prevent degradation. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Conforms to reference standard in phosphatase assay
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.