Description

4’-Methylumbelliferyl 2,3,4,-Tri-o-Acetyl-β-D-Glucopyranoside is a specialized fluorogenic substrate used in enzymatic assays. This compound is critical for researchers and manufacturers requiring high-purity, reliable biochemical tools for diagnostic and research applications. It is primarily utilized in the life sciences, pharmaceutical development, and clinical diagnostics industries for the detection and quantification of specific glycosidase enzyme activities.

Application

• Enzymatic Substrate for Glycosidases: Acts as a specific fluorogenic substrate for β-glucosidase and related enzymes, releasing the fluorescent 4-methylumbelliferone upon cleavage.
• Diagnostic Kit Manufacturing: A key raw material in the production of diagnostic kits for inborn errors of metabolism, such as Gaucher disease and other lysosomal storage disorders.
• Biochemical Research: Used in high-throughput screening (HTS) assays and enzyme kinetics studies to identify inhibitors or activators of target enzymes.
• Microbiology and Cell Biology: Employed in assays to study microbial enzyme activity or to develop reporter systems in cellular models.
• Pharmaceutical Quality Control: Utilized in potency testing and quality control of enzyme-based therapeutics.
• Food & Environmental Analysis: Applied in methods to detect specific enzymatic activities indicative of microbial contamination or process efficiency.

Basic Information

Product Name	4’-Methylumbelliferyl 2,3,4,-Tri-o-Acetyl-β-D-Glucopyranoside
CAS No.	937018-36-5
Molecular Formula	C₂₂H₂₄O₁₂
Molecular Weight	480.42 g/mol
Synonyms	4-MU-β-Glc-3Ac; 4-Methylumbelliferyl 2,3,4-tri-O-acetyl-β-D-glucopyranoside; 4-Methylumbelliferyl-β-D-glucoside triacetate; 4-MU-β-D-Glucopyranoside triacetate; 4-Methylumbelliferyl β-D-glucopyranoside 2',3',4'-triacetate; MU-β-Glc(Ac)₃; Fluorogenic β-glucosidase substrate triacetate
EINECS	Contact for details

Quality Control

Our 4’-Methylumbelliferyl 2,3,4,-Tri-o-Acetyl-β-D-Glucopyranoside is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Fluorogenic Activity	Functional in standard β-glucosidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.