Description

4’-Methylumbelliferyl 2,3,4,-Tri-o-Acetyl-6-o-Trityl-β-D-Glucopyranoside is a specialized fluorogenic glycoside derivative, primarily valued as a critical synthetic intermediate and substrate in enzymatic research. This compound is essential for the development and validation of high-sensitivity assays targeting glucosidase and related hydrolytic enzymes. It is a key material for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors who require precise, high-purity biochemical tools.

Application

• Enzyme Substrate: Acts as a fluorogenic substrate for the assay of β-glucosidase and other glycosidase enzymes, enabling sensitive detection of enzymatic activity.
• Biochemical Research: Serves as a crucial intermediate in the synthesis of more complex fluorogenic probes and labeled saccharides for studying carbohydrate metabolism.
• Diagnostic Development: Used in the research and production of diagnostic kits for genetic disorders like Gaucher's disease, where glucocerebrosidase activity is measured.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential inhibitors or activators of glycoside hydrolases.
• Glycobiology Studies: A valuable tool for investigating enzyme kinetics, mechanism of action, and substrate specificity within glycoscience research.

Basic Information

Product Name	4’-Methylumbelliferyl 2,3,4,-Tri-o-Acetyl-6-o-Trityl-β-D-Glucopyranoside
CAS No.	937018-35-4
Molecular Formula	C48H44O12
Molecular Weight	812.86 g/mol
Synonyms	4-MU-2,3,4-Tri-O-acetyl-6-O-trityl-β-D-glucopyranoside; 4-Methylumbelliferyl 2,3,4-tri-O-acetyl-6-O-trityl-β-D-glucopyranoside; 6-O-Trityl-4-methylumbelliferyl 2,3,4-tri-O-acetyl-β-D-glucopyranoside; 4-MU-Glc-6-O-Trityl triacetate; Fluorogenic β-glucosidase substrate intermediate; 4-Methylumbelliferyl β-D-glucopyranoside derivative
EINECS	Contact for details

Quality Control

Every batch of 4’-Methylumbelliferyl 2,3,4,-Tri-o-Acetyl-6-o-Trityl-β-D-Glucopyranoside is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with exacting standards for research and diagnostic applications. A detailed Certificate of Analysis (COA) providing purity, identity, and impurity profiles via HPLC, NMR, and MS is supplied with each shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.