Description

2-[Nα-Benzoylbenzoicamido-N6-(6-Biotinamidocaproyl)-L-Lysinylamido]Ethyl Methanethiosulfonate is a sophisticated, heterobifunctional crosslinking reagent designed for advanced bioconjugation and protein labeling applications. Its core value lies in its dual functionality, combining a methanethiosulfonate (MTS) group for specific cysteine modification with a biotin moiety for subsequent detection or purification via streptavidin. This compound is essential for researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development who require precise, site-specific protein tagging and immobilization.

Application

• Site-Directed Protein Labeling: Covalent attachment of biotin tags to specific cysteine residues in proteins for detection and pull-down assays.
• Surface Immobilization: Functionalization of biosensor chips, microarrays, and solid supports with biotinylated biomolecules.
• Antibody-Drug Conjugate (ADC) Research: A tool compound for exploring novel linker-payload chemistries in bioconjugate development.
• Structural Biology: Introduction of biotin probes for cryo-EM or other structural analysis techniques requiring specific labeling.
• Diagnostic Reagent Development: Creation of biotinylated detection antibodies or antigens for ELISA and lateral flow assays.
• Affinity Chromatography Ligand Synthesis: Preparation of biotinylated small molecules or peptides for streptavidin-column-based purification.

Basic Information

Product Name	2-[Nα-Benzoylbenzoicamido-N6-(6-Biotinamidocaproyl)-L-Lysinylamido]Ethyl Methanethiosulfonate
CAS No.	910036-44-1
Molecular Formula	C46H64N8O9S2
Molecular Weight	937.18 g/mol
Synonyms	Biotin-PEG3-MTS; Biotinylated Methanethiosulfonate Reagent; Biotin-(AC5)2-LC-MTS; N-((6-(Biotinamido)hexanoyl)-L-lysyl)-N-(2-(methylsulfonylthio)ethyl)-2-benzamidobenzamide; Biotin-LC-Lys(Mts)-BzBz; Heterobifunctional Biotin-MTS Crosslinker; Biotin Caproyl Lysine MTS; Benzoylbenzoyl Biotin Caproyl Lysine MTS
EINECS	Contact for details

Quality Control

Every batch of our high-purity biochemical reagents is manufactured and analyzed under strict quality protocols. Certificates of Analysis (COA) are provided, detailing key parameters such as purity (by HPLC), identity confirmation (by MS and/or NMR), and functional performance. We support research and development requiring reproducible, high-quality conjugation reagents and can accommodate custom purity specifications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.