Description

n-(3-Azidopropyl)Biotinamide is a specialized biotin derivative featuring a reactive azido group, designed for advanced bioconjugation and labeling applications. This compound is engineered to facilitate efficient, site-specific covalent attachment to biomolecules via click chemistry, offering researchers a powerful tool for probing biological systems. It is primarily utilized by scientists and manufacturers in the fields of biochemical research, proteomics, and diagnostic assay development.

Application

• Bioconjugation Reagent: For the site-specific labeling of proteins, antibodies, and other biomolecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry.
• Proteomics Research: Used in affinity purification, pull-down assays, and mass spectrometry to study protein-protein interactions and post-translational modifications.
• Diagnostic Development: Serves as a key component in the development of sensitive ELISA, lateral flow, and other immunoassay platforms requiring stable biotin-streptavidin linkages.
• Cell Surface Labeling: Enables the tagging and visualization of cell surface receptors and other membrane proteins for flow cytometry and microscopy.
• Material Science Functionalization: Used to modify surfaces of nanoparticles, polymers, and chips with biotin for creating biosensor interfaces.
• Chemical Biology Probes: Acts as a building block for synthesizing activity-based probes to study enzyme function and metabolic pathways.

Basic Information

Product Name	n-(3-Azidopropyl)Biotinamide
CAS No.	908007-17-0
Molecular Formula	C13H22N6O2S
Molecular Weight	326.42 g/mol
Synonyms	Biotin-PEG3-Azide; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-(3-azidopropyl)pentanamide; N-(3-Azidopropyl)-5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanamide; Biotin-azide; Azido-PEG3-biotin; Biotin-PEG3-N3; Biotin Azide; Biotinylated Azide
EINECS	Contact for details

Quality Control

Our n-(3-Azidopropyl)Biotinamide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance. Certificates of Analysis (COA) detailing specifications such as purity (by HPLC), identity (by NMR and MS), and functional group integrity are provided to guarantee the material meets the stringent requirements for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. The compound is light-sensitive (store away from light). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.