Description

6-Hexadecanoylamino-4-Methylumbelliferyl Phosphorylcholine is a specialized fluorogenic substrate designed for the sensitive detection and assay of phospholipase activity, particularly phospholipase D (PLD). This compound matters as a critical research tool for studying lipid signaling pathways, membrane dynamics, and enzyme kinetics in biochemical and pharmacological research. It is primarily needed by scientists and quality control laboratories in the pharmaceutical, biotechnology, and academic research sectors for high-throughput screening and diagnostic assay development.

Application

• Enzyme Activity Assays: Primary substrate for the fluorometric detection and quantification of phospholipase D (PLD) and related phospholipase enzymes.
• Biochemical Research: Used in studies of lipid metabolism, cell signaling pathways, and second messenger systems involving phosphatidic acid.
• Drug Discovery & High-Throughput Screening (HTS): Employed in pharmaceutical R&D for screening potential PLD inhibitors or activators as therapeutic targets for cancer, inflammatory, and neurodegenerative diseases.
• Diagnostic Development: Serves as a key component in developing clinical diagnostic kits for conditions associated with abnormal phospholipase activity.
• Cell Biology Studies: Applied in live-cell or cell lysate-based imaging and assays to visualize and measure localized PLD activity.

Basic Information

Product Name	6-Hexadecanoylamino-4-Methylumbelliferyl Phosphorylcholine
CAS No.	904315-61-3
Molecular Formula	C₃₁H₅₃N₂O₉P
Molecular Weight	628.74 g/mol
Synonyms	4-Methylumbelliferyl Phosphorylcholine Hexadecanoylamino Derivative; Amplex Red PLD Substrate Analog; Hexadecanoyl-AMC-PC; PLD Fluorogenic Substrate; 6-Palmitoylamino-4-Methylumbelliferyl Phosphorylcholine; N-Hexadecanoyl-6-amino-4-methylumbelliferyl Phosphorylcholine; Fluorogenic Phospholipase D Substrate; 6-(Palmitoylamino)-4-methyl-2-oxo-2H-chromen-7-yl 2-(trimethylazaniumyl)ethyl phosphate
EINECS	Contact for details

Quality Control

Our production of 6-Hexadecanoylamino-4-Methylumbelliferyl Phosphorylcholine adheres to stringent quality protocols suitable for research and diagnostic applications. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation. Certificates of Analysis (COA) detailing purity, identity, and performance in standard assay conditions are available upon request to ensure traceability and reliability for your critical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffer
Functional Activity	Suitable for fluorometric PLD assay (typical λex/λem ~360/450 nm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.