Description

Chlorophenol Red-β-D-Galactopyranosid is a chromogenic substrate used for the detection of β-galactosidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by biotechnology, pharmaceutical, and life science research industries for assays in molecular biology, microbiology, and cell biology.

Application

• β-Galactosidase Enzyme Assays: As a chromogenic substrate in quantitative and qualitative enzymatic activity tests.
• Molecular Biology Research: Used in reporter gene assays (e.g., lacZ gene expression) to monitor gene regulation and promoter activity.
• Microbiological Testing: Employed in media for the identification of microorganisms based on β-galactosidase production.
• Cell Biology & Histochemistry: A tool for visualizing β-galactosidase expression in fixed cells and tissues.
• High-Throughput Screening (HTS): Suitable for developing colorimetric assays in drug discovery platforms.
• Diagnostic Kit Component: Used in the formulation of diagnostic kits for enzymatic deficiencies or bacterial identification.

Basic Information

Product Name	Chlorophenol Red-β-D-Galactopyranosid
CAS No.	99792-79-7
Molecular Formula	C25H21ClO9S
Molecular Weight	532.94 g/mol
Synonyms	Chlorophenol Red-β-D-Galactopyranoside; CPRG; 3'-Chloro-3''-[(3,3-dimethyl-5-sulfo-1,1-dioxo-1,2-benzoxathiol-7-yl)azo]-4'-hydroxy-5'-methoxyfuchson-5,5'-diyl bis(β-D-galactopyranoside); Chlorophenolred-β-D-galactopyranoside; β-Galactosidase Substrate CPRG; 5-Chloro-3-indolyl-β-D-galactopyranoside analog (chromogenic)
EINECS	Contact for details

Quality Control

Our Chlorophenol Red-β-D-Galactopyranosid is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance in enzymatic assays. Certificates of Analysis (COA) detailing purity, identity, and performance specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is kept sealed in a desiccated environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	Reddish-orange to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Solubility	Soluble in water, DMSO
Enzymatic Activity	Suitable for β-galactosidase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.