Description

Chlorophenolred-β-D-Galactopyranoside, Sodium Salt is a chromogenic substrate specifically designed for the detection and quantification of β-galactosidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by professionals in biochemical research, molecular biology, and diagnostic kit manufacturing for applications such as gene expression studies and microbial detection.

Application

• Biochemical Research: As a chromogenic substrate for β-galactosidase in enzyme kinetics and inhibition studies.
• Molecular Biology: Used in reporter gene assays (e.g., lacZ gene) to monitor gene expression and promoter activity.
• Diagnostic Assays: A key component in clinical and microbiological test kits for detecting bacterial enzymes, such as in E. coli identification.
• Cell Biology: For staining applications in histochemistry and cytometry to visualize β-galactosidase activity in cells and tissues.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential modulators of β-galactosidase activity.
• Food & Environmental Testing: Used in assays to detect specific microbial contamination based on galactosidase activity.

Basic Information

Product Name	Chlorophenolred-β-D-Galactopyranoside, Sodium Salt
CAS No.	99792-50-4
Molecular Formula	C25H27ClN2O9S · xNa
Molecular Weight	Approx. 575.0 (for free acid, C25H28ClN2O9S)
Synonyms	CPRG, Sodium Salt; Chlorophenol Red-β-D-Galactopyranoside, Sodium Salt; 5-Chloro-3-[3-(β-D-galactopyranosyloxy)-2,4,6-triiodophenyl]-2-hydroxybenzenesulfonic Acid Sodium Salt; β-Galactosidase Substrate CPRG; CPRG Na Salt; Chromogenic Galactosidase Substrate; LacZ Substrate CPRG.
EINECS	Contact for details

Quality Control

Our Chlorophenolred-β-D-Galactopyranoside, Sodium Salt is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, performance in enzyme assays, and other critical parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Reddish-orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO
Functional Activity (Enzyme Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.