Description

Benzyloxycarbonylphenylalanine 4-Methylcoumaryl-7-Ester is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of protease activity, particularly chymotrypsin and related enzymes. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic reagent development sectors for enzyme kinetics studies, inhibitor screening, and assay development.

Application

• Protease Activity Assays: As a sensitive fluorogenic substrate for chymotrypsin and other serine proteases.
• Enzyme Kinetics & Inhibitor Screening: Used in high-throughput screening (HTS) platforms to study enzyme mechanisms and identify potential therapeutic inhibitors.
• Diagnostic Reagent Development: A key component in the formulation of diagnostic kits for detecting specific enzymatic activities in clinical samples.
• Biochemical Research: Employed in academic and industrial R&D for studying protein function, signal transduction pathways, and proteolytic processing.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity and purity of enzyme preparations in pharmaceutical production.

Basic Information

Product Name	Benzyloxycarbonylphenylalanine 4-Methylcoumaryl-7-Ester
CAS No.	99678-18-9
Molecular Formula	C27H23NO6
Molecular Weight	457.48 g/mol
Synonyms	Z-Phe-MCA; Cbz-Phe-MCA; Benzyloxycarbonyl-L-phenylalanine 7-amido-4-methylcoumarin; Z-Phe-AMC; N-Carbobenzoxy-L-phenylalanine 4-methylcoumaryl-7-amide; Chymotrypsin Substrate I (Fluorogenic); Protease Substrate; L-Phenylalanine, N-[(phenylmethoxy)carbonyl]-, 4-methyl-2-oxo-2H-1-benzopyran-7-yl ester
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylphenylalanine 4-Methylcoumaryl-7-Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.