Description

25-Hydroxyvitamin D3 3-Sulfate Ester is a key sulfated metabolite of vitamin D3, playing a significant role in the body's calcium and phosphate homeostasis. This compound is of high interest for research into vitamin D metabolism, transport, and storage mechanisms. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic manufacturers, and academic institutions for use as a critical reference standard and a precursor in biochemical studies.

Application

• Pharmaceutical Reference Standard: Used for the calibration and validation of analytical methods (HPLC, LC-MS/MS) in drug development and quality control.
• Clinical Diagnostics: Serves as a critical calibrator and control material in the development of immunoassays and mass spectrometry-based tests for measuring vitamin D status.
• Biochemical Research: Employed in studies investigating the sulfation pathway of vitamin D metabolism, its biological activity, and receptor interactions.
• Nutritional Science: Used in research to understand the bioavailability and physiological role of sulfated vitamin D metabolites.
• Metabolite Synthesis: Acts as a key intermediate or precursor for the synthesis of other vitamin D analogs and metabolites.

Basic Information

Product Name	25-Hydroxyvitamin D3 3-Sulfate Ester
CAS No.	99447-30-0
Molecular Formula	C27H44O5S
Molecular Weight	480.71 g/mol
Synonyms	25-Hydroxycholecalciferol 3-sulfate; 25-Hydroxyvitamin D3 sulfate; (3S,5Z,7E)-9,10-Secocholesta-5,7,10(19)-trien-3,25-diol 3-sulfate; 25(OH)D3-S; Calcifediol 3-sulfate; 25-Hydroxyvitamin D3 3-sulfate; 25-Hydroxyvitamin D3 sulphate ester
EINECS	Contact for details

Quality Control

Our 25-Hydroxyvitamin D3 3-Sulfate Ester is produced under strict quality management systems. Each batch is subjected to rigorous analytical testing, including HPLC and LC-MS/MS, to ensure high purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and related substances are provided with every shipment to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/LC-MS)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.