Description

Onozuka P 500 is a high-purity enzyme preparation derived from *Trichoderma viride*, widely recognized for its specific cellulase activity. This product is essential for the controlled degradation of cellulose in research and industrial bioprocessing applications. It is a critical reagent for biotechnology, pharmaceutical, and life science laboratories requiring precise enzymatic activity for cell wall digestion and biomass conversion.

Application

• Plant Protoplast Isolation: Key enzyme for digesting plant cell walls to release viable protoplasts for genetic engineering and cell culture studies.
• Biomass Saccharification: Used in research-scale processes to break down cellulose in agricultural waste into fermentable sugars for biofuel production.
• Cell Wall Component Analysis: Employed in biochemical research to study cellulose structure and composition in various plant materials.
• Enzymatic Activity Studies: Serves as a standard or reference cellulase preparation in enzyme kinetics and inhibition assays.
• Tissue Maceration: Facilitates the gentle separation of plant tissues for histological and microbiological examination.
• Food & Feed Research: Investigated for improving digestibility and nutritional value of fibrous materials in feed and food processing research.

Basic Information

Product Name	Onozuka P 500
CAS No.	99331-82-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cellulase from Trichoderma viride; Cellulase AP; Cellulase Onozuka; Macerozyme R-10 (related preparation); Cellulysin; Trichoderma viride cellulase; 1,4-(1,3;1,4)-β-D-Glucan 4-glucanohydrolase (Trichoderma viride); Cellulase P 500
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is characterized for specific enzymatic activity and purity profiles relevant to research applications. Certificates of Analysis (COA) detailing activity units, protein content, and absence of specified contaminants are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C under desiccant.

Specification

Item	Specification
Appearance	Light brown to yellow powder or lyophilized solid
Identification (Activity)	Conforms (Cellulase Activity)
Protein Content (Biuret)	≥ 50%
Activity (Filter Paper Units/g)	Contact for details
pH (in solution)	~ 5.0 - 7.0
Solubility	Soluble in water, buffer solutions
Loss on Drying	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.