Description

Proteinase K is a highly active, broad-spectrum serine protease widely recognized for its exceptional stability and ability to digest native proteins. This enzyme is critical for applications requiring the efficient inactivation of nucleases and the degradation of contaminating proteins without damaging sensitive nucleic acids. It is an indispensable reagent for molecular biology, diagnostic, and biopharmaceutical industries, where high-purity enzymatic activity is paramount for reliable results.

Application

• Nucleic Acid Isolation & Purification: Essential for digesting proteins and inactivating RNases and DNases during DNA and RNA extraction from tissues, cells, and blood.
• Molecular Biology Research: Used in plasmid preparation, removal of contaminating enzymes (e.g., in PCR), and general protein digestion in buffer solutions.
• Pathogen Inactivation: Employed in virology and prion research for degrading proteinaceous infectious agents and reducing biohazard risks.
• Diagnostic Kit Manufacturing: A key component in diagnostic kits for infectious diseases and genetic testing, ensuring sample clarity and assay accuracy.
• Biopharmaceutical Processing: Used in downstream processing for cell lysis and clearance of host cell proteins during therapeutic protein and vaccine production.
• Forensic Analysis: Applied to digest proteins in forensic samples for subsequent DNA profiling and analysis.

Basic Information

Product Name	Proteinase K
CAS No.	39450-01-6
Molecular Formula	N/A (Enzyme)
Molecular Weight	~28,900 Da (Monomer)
Synonyms	Protease K; Endopeptidase K; Tritirachium album proteinase K; T. album serine protease; PK enzyme; Protease from Tritirachium album; Proteinase K from Tritirachium album; Serine protease (Tritirachium album)
EINECS	Contact for details

Quality Control

Our Proteinase K is manufactured under strict quality management systems. Each batch is tested to ensure high specific activity, purity, and absence of contaminating nuclease and protease activities. Certificates of Analysis (COA) detailing specifications such as activity units/mg, purity (SDS-PAGE), and stability are provided. We support compliance requirements for GMP, ISO 13485, and other relevant quality standards for critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C. Keep the container tightly sealed in a dry environment to prevent moisture absorption and loss of activity.

Specification

Item	Specification
Appearance	White to light brown lyophilized powder
Identification (IR)	Conforms
Activity	>30 units/mg protein
Purity (SDS-PAGE)	>95%
DNase & RNase Activity	Not detected
Protease Activity (contaminating)	Not detected
Solubility	Clear, slightly opalescent solution in water or buffer
Loss on Drying	<5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.