Description

Restriction Endonuclease Asn I is a highly specific type II restriction enzyme that recognizes and cleaves DNA at the palindromic sequence 5'-AT↓TAAT-3'. This precision tool is critical for molecular biology research, enabling the manipulation and analysis of genetic material with high fidelity. It is an essential reagent for biotechnology, pharmaceutical development, and academic laboratories engaged in genetic engineering, cloning, and genomic mapping.

Application

• Recombinant DNA Technology: For precise DNA digestion in cloning workflows to create recombinant plasmids.
• Genomic Analysis: Used in restriction mapping and Southern blotting to analyze gene structure and organization.
• Molecular Diagnostics: Employed in RFLP (Restriction Fragment Length Polymorphism) analysis for genetic fingerprinting and mutation detection.
• Biopharmaceutical Manufacturing: Critical in the quality control and characterization of plasmid DNA and viral vectors used in gene therapy and vaccine production.
• Synthetic Biology: Facilitates the assembly of genetic circuits and synthetic pathways by providing specific cleavage sites.
• Forensic Science: Aids in DNA profiling and analysis for identification purposes.

Basic Information

Product Name	Restriction Endonuclease Asn I
CAS No.	117164-98-4
Molecular Formula	Protein (Enzyme)
Molecular Weight	Contact for details
Synonyms	AsnI; Endodeoxyribonuclease AsnI; Restriction enzyme AsnI; Type II site-specific deoxyribonuclease AsnI; EC 3.1.21.4; Asn I (from Arthrobacter species); 5'-AT↓TAAT-3' specific endonuclease
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Asn I is produced under stringent quality management systems. Each batch is rigorously tested for specific activity, purity, and absence of contaminating nucleases (exonuclease and non-specific endonuclease) to ensure optimal performance in sensitive applications. Certificates of Analysis (COA) detailing lot-specific activity, storage buffer, and quality control assays are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in the supplied glycerol-containing storage buffer. Avoid repeated freeze-thaw cycles. Keep the enzyme on ice during use.

Specification

Item	Specification
Appearance	Clear colorless solution
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Exonuclease Activity	Not detectable
Non-specific Endonuclease Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4
Optimal Incubation Temperature	37°C
Heat Inactivation	65°C for 20 minutes

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.