Description

Acyloxyacyl Hydrolase is a specialized enzyme that catalyzes the hydrolysis of acyloxyacyl linkages, a key structural component found in certain bacterial lipopolysaccharides. This activity is of significant interest for its role in modulating innate immune responses and inflammation pathways. It serves as a critical research tool for immunologists, biochemists, and pharmaceutical developers working on novel anti-inflammatory therapeutics and vaccine adjuvants. The compound is supplied as a high-purity reagent to ensure reliable and reproducible experimental results.

Application

• Immunology Research: Used to study the detoxification of bacterial endotoxins (LPS) and the subsequent modulation of Toll-like receptor 4 (TLR4) signaling pathways.
• Inflammation Studies: A key reagent for investigating the biochemical mechanisms underlying sepsis, septic shock, and chronic inflammatory diseases.
• Vaccine Development: Employed in research to engineer safer vaccine adjuvants by modifying lipid A structures of LPS.
• Enzymology & Biochemistry: Serves as a model enzyme for studying the kinetics and specificity of serine hydrolases that act on complex lipid substrates.
• Pharmaceutical Screening: Utilized in high-throughput assays to identify and characterize potential inhibitors or activators of the enzyme for therapeutic intervention.
• Diagnostic Reagent Manufacturing: May be incorporated into diagnostic kits designed to detect or quantify specific bacterial contaminants or host inflammatory markers.

Basic Information

Product Name	Acyloxyacyl Hydrolase
CAS No.	110277-64-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Acyloxyacyl Hydrolase; AOAH; LPS Deacylase; Endotoxin Deacylating Enzyme; Lipid A Deacylase; EC 3.1.1.77; Acyloxyacyl Hydrolyase; Bacterial Lipopolysaccharide Acyloxyacyl Hydrolase
EINECS	Contact for details

Quality Control

Our Acyloxyacyl Hydrolase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot is characterized for specific activity, purity, and stability. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing results for identity, purity (by SDS-PAGE and/or HPLC), protein concentration, specific enzymatic activity, and absence of contaminating proteases. We adhere to relevant guidelines for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot into single-use vials after reconstitution to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Catalyzes hydrolysis of specific acyloxyacyl substrate
Purity (SDS-PAGE)	≥ 90%
Specific Activity	≥ [Value] units/mg protein
Protein Content (Bradford)	≥ [Value] mg/vial
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in appropriate aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.