Description

Ribonuclease A is a highly specific endoribonuclease that catalyzes the cleavage of single-stranded RNA at the 3'-end of pyrimidine nucleotides. This enzymatic precision makes it an indispensable tool for eliminating RNA contamination from biological samples, ensuring the integrity of downstream molecular biology applications. It is essential for research and production workflows in molecular biology, pharmaceutical development, and diagnostic manufacturing where pure DNA or protein samples are critical.

Application

• RNA Removal in DNA Purification: Critical for preparing RNA-free genomic DNA or plasmid DNA for PCR, sequencing, and cloning.
• Protein Sample Preparation: Used to digest RNA in cell lysates prior to protein purification techniques like chromatography or electrophoresis.
• Virology and Vaccine Research: Employed in the study of RNA viruses and the development of vaccines to process viral samples.
• Diagnostic Kit Manufacturing: A key reagent in diagnostic kits (e.g., PCR kits) to prevent false positives from RNA contamination.
• Biochemical Research: Used in studies of enzyme kinetics, nucleic acid-protein interactions, and RNA metabolism.
• Pharmaceutical Production: Applied in the downstream processing of biopharmaceuticals to remove host cell RNA.

Basic Information

Product Name	Ribonuclease A
CAS No.	9001-99-4
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	~13.7 kDa
Synonyms	RNase A; Pancreatic Ribonuclease; Ribonuclease I; Endoribonuclease A; EC 3.1.27.5; Benzonase (related function); RNA-digesting enzyme
EINECS	232-656-6

Quality Control

Our Ribonuclease A is manufactured and tested under stringent quality systems. Each batch is characterized for specific activity, purity, and the absence of contaminating DNase and protease activities. We provide Certificates of Analysis (COA) with detailed specifications, including activity units/mg and purity by SDS-PAGE. Production can adhere to relevant guidelines for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive). For extended storage, consider desiccant. Avoid repeated freeze-thaw cycles of prepared solutions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms (Specific Activity ≥ 50 Kunitz units/mg protein)
Purity (SDS-PAGE)	≥ 90%
DNase Activity	Not detected
Protease Activity	Not detected
Solubility	Soluble in water or buffer (10 mM Tris-HCl, pH 7.5)
Protein Content (Biuret)	≥ 70%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.