Description

Ceramide Glycanase is a specialized enzyme that catalyzes the hydrolysis of glycosidic linkages in glycosphingolipids, specifically cleaving the glycan moiety from ceramide. This precise biocatalytic activity is critical for research and development in glycobiology and lipid metabolism studies. It is an essential tool for pharmaceutical, biotechnology, and academic laboratories focused on neurodegenerative disease research, biomarker discovery, and the synthesis of bioactive sphingolipid derivatives.

Application

• Glycobiology Research: Used for the structural analysis and sequencing of complex glycosphingolipids (GSLs) to understand their biological functions.
• Biomarker Discovery: Aids in the enzymatic digestion of GSLs from biological samples for subsequent mass spectrometric analysis to identify disease-specific markers.
• Pharmaceutical Development: Employed in the study of lysosomal storage disorders (e.g., Gaucher's disease, Fabry disease) where glycosphingolipid metabolism is disrupted.
• Enzyme Mechanism Studies: Serves as a model enzyme for investigating the catalytic mechanisms of glycan hydrolases within the ceramide glycanase family.
• Synthetic Biology: Utilized in the chemoenzymatic synthesis of defined glycosphingolipid analogs for use as standards, probes, or potential therapeutic agents.
• Diagnostic Reagent Formulation: A key component in enzymatic assay kits designed for the quantitative measurement of specific glycosphingolipids in clinical samples.

Basic Information

Product Name	Ceramide Glycanase
CAS No.	106769-66-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Glycosylceramidase; Glycosylceramide Glycanohydrolase; Ceramide Glycosidase; GSL Glycanase; Glycosphingolipid Ceramide Deacylase; Glycolipid Ceramide Deacylase; E.C. 3.2.1.?? (Contact for specific EC number)
EINECS	Contact for details

Quality Control

Our Ceramide Glycanase is produced and tested under stringent quality management protocols. Each batch is characterized for specific activity, purity, and absence of contaminating protease or glycosidase activities. Certificates of Analysis (COA) detailing assay results, purity by SDS-PAGE, and stability data are provided to ensure the product meets the exacting standards required for critical research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product is typically supplied as a lyophilized powder or in a buffered glycerol solution; refer to the specific product label or COA for precise storage instructions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (Activity)	Hydrolyzes specific glycosphingolipid substrate
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content	By Bradford or UV absorbance
Contaminating Activities	Free of detectable protease and non-specific glycosidase activities
pH (in solution)	7.0 - 7.5
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.