Description

Restriction Endonuclease Snab I From *Sph Aerotilus N is a highly specific type II restriction enzyme that recognizes and cleaves DNA at a particular palindromic sequence. This precision tool is critical for molecular biology research, enabling the manipulation and analysis of genetic material with high fidelity. It is an essential reagent for biotechnology companies, academic research laboratories, and diagnostic kit manufacturers engaged in genetic engineering, cloning, and DNA mapping.

Application

• Molecular Cloning: Essential for cutting DNA at specific sites to insert foreign DNA fragments into vectors.
• Genetic Engineering: Used in the construction of recombinant DNA molecules for gene expression studies and protein production.
• DNA Mapping (RFLP Analysis): Key for generating restriction fragment length polymorphisms used in genetic fingerprinting and mutation detection.
• Genome Assembly & Sequencing: Facilitates the fragmentation of genomic DNA into manageable pieces for next-generation sequencing projects.
• Diagnostic Assay Development: Employed in the design of tests that rely on specific DNA cleavage patterns to identify pathogens or genetic disorders.
• Plasmid Construction & Validation: Critical for verifying plasmid integrity and ensuring correct insert orientation during synthetic biology workflows.

Basic Information

Product Name	Restriction Endonuclease Snab I From *Sph Aerotilus N
CAS No.	103780-21-8
Molecular Formula	Protein (Enzyme)
Molecular Weight	Contact for details
Synonyms	Snab I Restriction Enzyme; Endodeoxyribonuclease Snab I; SnabI; Restriction Enzyme Snab I; *Sph Aerotilus N Restriction Endonuclease; Type II Restriction Enzyme Snab I; EC 3.1.21.4 (related class)
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Snab I is produced and tested under stringent conditions to ensure high activity, specificity, and absence of contaminating nucleases. Each batch is validated for its precise cleavage pattern and optimal performance in standard buffer systems. Certificates of Analysis (COA) detailing activity units, purity, and stability are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. The enzyme is supplied in a storage buffer containing glycerol. Avoid repeated freeze-thaw cycles. Aliquoting upon receipt is recommended for frequent use.

Specification

Item	Specification
Appearance	Clear colorless solution
Activity (Units/µl)	≥ 10,000
Purity (SDS-PAGE)	≥ 95%
Specificity	Cleaves at recognized palindromic sequence only
Contaminating Nuclease Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4
Optimal Incubation Temperature	37°C
Heat Inactivation	65°C for 20 minutes

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.