Description

Proteinase K is a highly active, broad-spectrum serine protease widely used for its exceptional ability to digest proteins and inactivate nucleases. This robust enzymatic activity is critical for ensuring the purity and integrity of nucleic acids in downstream molecular biology applications. It is an indispensable reagent for research, diagnostic, and biopharmaceutical laboratories requiring reliable sample preparation.

Application

• Nucleic Acid Isolation & Purification: Digestion of contaminating proteins during DNA and RNA extraction from tissues, cells, or bacteria.
• Molecular Biology Research: Protein removal in plasmid preparation, genomic DNA isolation, and inactivation of RNases and DNases.
• Pathogen Detection & Diagnostics: Sample preparation for PCR, qPCR, and next-generation sequencing (NGS) by lysing viral envelopes and degrading cellular proteins.
• Forensic Analysis: Digestion of proteins in forensic samples to release nucleic acids for genetic fingerprinting.
• Biopharmaceutical Manufacturing: Used in processes requiring the clearance of host cell proteins and other process-related impurities.
• General Protein Digestion: A versatile tool for degrading proteins in solution prior to various analytical techniques.

Basic Information

Product Name	Proteinase K
CAS No.	102925-54-2
Molecular Formula	N/A (Enzyme)
Molecular Weight	~28.9 kDa (Monomer)
Synonyms	Protease K; Endopeptidase K; Tritirachium album proteinase K; T. album alkaline proteinase; PK; Proteínasa K
EINECS	Contact for details

Quality Control

Our Proteinase K is manufactured and tested under a stringent quality management system. Each lot is characterized for critical parameters including specific activity, purity, and absence of contaminating nuclease activities. We provide comprehensive Certificates of Analysis (COA) detailing assay results, ensuring the product meets the rigorous demands of life science research and regulated applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	White to light brown lyophilized powder
Identification (Activity)	Conforms
Specific Activity	≥ 30 units/mg protein
Protein Content (Biuret)	Contact for details
DNase Activity	Not detectable
RNase Activity	Not detectable
Solubility	Clear, slightly opalescent solution in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.