Description

Succinyl-Tyrosyl-Leucyl-Phenylalanine-4-Nitroanilide is a synthetic chromogenic peptide substrate specifically designed for enzymatic assays. This compound is a critical reagent for the quantitative determination of chymotrypsin and related serine protease activity, offering high sensitivity and specificity. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies, inhibitor screening, and purity testing of proteolytic enzymes.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric measurement of α-chymotrypsin, elastase, and other serine proteases.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for measuring pancreatic function and other clinical markers.
• Biochemical Research: Essential for studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial R&D settings.
• Quality Control (QC) Testing: Employed in QC laboratories to assess the potency and purity of enzyme preparations, including those used in industrial processes.
• Proteomics: Used as a tool in proteomic studies to analyze protease activity profiles in complex biological samples.

Basic Information

Product Name	Succinyl-Tyrosyl-Leucyl-Phenylalanine-4-Nitroanilide
CAS No.	99242-09-8
Molecular Formula	C34H40N6O9
Molecular Weight	676.72 g/mol
Synonyms	Suc-Tyr-Leu-Phe-pNA; Suc-YLF-pNA; N-Succinyl-L-Tyrosyl-L-Leucyl-L-Phenylalanine 4-Nitroanilide; Chymotrypsin Substrate IV; Suc-Tyr-Leu-Phe-p-Nitroanilide; N-Succinyl-Tyrosine-Leucine-Phenylalanine-p-Nitroanilide; Protease Substrate Suc-YLF-pNA
EINECS	Contact for details

Quality Control

Our Succinyl-Tyrosyl-Leucyl-Phenylalanine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.