Description

3-(n-Maleimidopropionyl)Biocytin is a heterobifunctional crosslinking reagent that combines a maleimide group for thiol conjugation with a biocytin moiety for avidin/streptavidin-based detection. This compound is essential for creating stable, site-specific bioconjugates, enabling precise labeling and purification of biomolecules. It is primarily used by researchers and developers in bioconjugation, proteomics, and diagnostic assay development, where reliable and specific protein tagging is required.

Application

• Bioconjugation and Protein Labeling: Site-specific conjugation of peptides, antibodies, or other proteins via free thiol groups for creating detection probes.
• Affinity Purification and Pull-Down Assays: Immobilization of target proteins onto streptavidin/avidin resins for isolation and complex analysis.
• Diagnostic Assay Development: Used in the development of ELISA, lateral flow assays, and other immunoassays for signal generation and amplification.
• Cell Surface Labeling: Tagging of cell surface thiol groups for fluorescence-activated cell sorting (FACS) or microscopy studies.
• Proteomics Research: Crosslinking agent for studying protein-protein interactions and mapping structural domains.
• Drug Delivery System Conjugation: Linking therapeutic agents or targeting ligands to carrier systems via cleavable or stable linkages.

Basic Information

Product Name	3-(n-Maleimidopropionyl)Biocytin
CAS No.	98930-71-3
Molecular Formula	C25H41N7O8S
Molecular Weight	599.70 g/mol
Synonyms	N-(3-Maleimidopropionyl)biocytin; Maleimidopropionyl Biocytin; Biocytin Maleimide; Biocytin-3-maleimidopropionamide; 3-Maleimidopropionic Acid Biocytin Amide; N-ε-Biotinyl-L-lysine 3-Maleimidopropionamide; MPB-Biotin; Biotin Maleimide (C3 linker)
EINECS	Contact for details

Quality Control

Our 3-(n-Maleimidopropionyl)Biocytin is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the standards required for research-grade and in-vitro diagnostic (IVD) component manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water
Functional Activity	Conjugation efficiency confirmed by model thiol assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.