Description

1,25-Dihydroxyvitamin D3-3-(n-(4-Azido-2-Nitrophenyl)Glycinate) is a chemically modified, photoactivatable derivative of the biologically active form of Vitamin D, Calcitriol. This compound matters as a critical biochemical tool for studying Vitamin D receptor (VDR) signaling pathways, enabling researchers to cross-link and isolate receptor complexes. It is primarily needed by pharmaceutical R&D teams, academic research institutions, and biotechnology companies focused on endocrinology, bone metabolism, and cancer research.

Application

• Photoaffinity Labeling: Acts as a covalent probe for mapping the binding sites and interactions of the Vitamin D receptor (VDR) and its associated proteins.
• Biochemical Research: Used to study the mechanisms of action, metabolism, and downstream signaling of 1,25-dihydroxyvitamin D3 (Calcitriol).
• Drug Discovery: Serves as a key intermediate or probe in the development of novel Vitamin D analogs for therapeutic applications.
• Molecular Biology: Facilitates the isolation and purification of VDR complexes from cell lysates for proteomic analysis.
• Endocrinology Studies: Enables detailed investigation into the role of Vitamin D in calcium homeostasis, immune function, and cell differentiation.

Basic Information

Product Name	1,25-Dihydroxyvitamin D3-3-(n-(4-Azido-2-Nitrophenyl)Glycinate)
CAS No.	98728-28-0
Molecular Formula	C₃₃H₄₄N₄O₇
Molecular Weight	620.73 g/mol
Synonyms	Calcitriol 3-(N-(4-Azido-2-nitrophenyl)glycinate); 1α,25-Dihydroxycholecalciferol 3-(4-Azido-2-nitrophenylglycinate); Photoaffinity Labeling Analog of 1,25-(OH)₂D₃; Vitamin D3 Photoaffinity Probe; 1,25-Dihydroxyvitamin D3 ANP-Glycinate; 1,25(OH)₂D₃-3-ANG; [³H]-1,25-Dihydroxyvitamin D3-3-(N-(4-azido-2-nitrophenyl)glycinate) (for radiolabeled form)
EINECS	Contact for details

Quality Control

Our 1,25-Dihydroxyvitamin D3-3-(n-(4-Azido-2-Nitrophenyl)Glycinate) is produced under strict quality protocols suitable for research applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure consistent performance and reliability. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its hygroscopic and strictly light-sensitive nature, the material should be handled in a controlled environment with minimal exposure to moisture and light. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, ethanol, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.