Description

Bis(2,5-Dioxopyrrolidin-1-Yl) 4,4’-Disulfanediyldibutanoate is a specialized heterobifunctional crosslinking agent featuring an amine-reactive NHS ester group and a cleavable disulfide bond. This compound is engineered for the controlled conjugation of biomolecules, enabling the formation of reversible linkages that are critical for advanced bioconjugation strategies. It is essential for researchers and manufacturers in the fields of biopharmaceutical development, proteomics, and diagnostic assay production who require precise and reversible protein-protein or protein-ligand crosslinking.

Application

• Bioconjugation Reagent: For the site-specific crosslinking of peptides, proteins, and antibodies, facilitating the creation of antibody-drug conjugates (ADCs) and other therapeutic bioconjugates.
• Proteomics Research: Used in the study of protein-protein interactions and complex formation, where the cleavable disulfide bond allows for the recovery of linked components under reducing conditions.
• Diagnostic Kit Manufacturing: Employed in the development of enzyme-linked immunosorbent assays (ELISAs) and other immunoassays to conjugate detection enzymes to antibodies.
• Biomaterial Functionalization: For modifying surfaces of nanoparticles, polymers, or chips with bioactive molecules to create smart drug delivery systems or biosensors.
• Affinity Chromatography Ligand Synthesis: In the preparation of solid-phase media where ligands are attached via a reversible linkage for gentle elution.
• Stable Intermediate in Organic Synthesis: Serves as a key building block for synthesizing more complex molecules with specific reactive handles in medicinal chemistry.

Basic Information

Product Name	Bis(2,5-Dioxopyrrolidin-1-Yl) 4,4’-Disulfanediyldibutanoate
CAS No.	98604-88-7
Molecular Formula	C16H20N2O8S2
Molecular Weight	440.47 g/mol
Synonyms	NHS-SS-NHS; 3,3'-Dithiobis(propionic acid N-hydroxysuccinimide ester); DTSSP (Dithiobis(succinimidyl propionate)); Disuccinimidyl 3,3'-dithiodipropionate; Bis(N-succinimidyl) 3,3'-dithiodipropionate; 1,1'-[Dithiobis(2,1-ethanediyl-1-oxo-3,1-propanediyl)]bis[2,5-pyrrolidinedione]; Succinimidyl 3,3'-dithiodipropionate; SDPD
EINECS	Contact for details

Quality Control

Our Bis(2,5-Dioxopyrrolidin-1-Yl) 4,4’-Disulfanediyldibutanoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to prevent hydrolysis of the NHS ester groups. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.