Description

L-Phe-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate and a key biochemical building block. This compound is critically important for its role as a selective and sensitive probe in enzymatic assays, particularly for proteases like chymotrypsin and subtilisin. It is primarily needed by research institutions, pharmaceutical developers, and diagnostic kit manufacturers engaged in high-throughput screening, enzyme kinetics studies, and the development of novel therapeutic and diagnostic platforms.

Application

• Protease Activity Assays: A premier fluorogenic substrate for continuous monitoring of chymotrypsin, subtilisin, and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize protease inhibitors or activators.
• Biochemical Research: Essential for fundamental studies on enzyme mechanism, kinetics, and specificity.
• Diagnostic Development: Serves as a core component in the design of sensitive diagnostic tests for pathological conditions involving protease activity.
• Peptide Synthesis Intermediate: Acts as a protected or modified amino acid derivative in the synthesis of more complex peptide-based probes.
• Cell Biology Studies: Employed in assays to detect and localize protease activity within cellular systems.

Basic Information

Product Name	L-Phe-7-Amino-4-Methylcoumarin
CAS No.	98516-72-4
Molecular Formula	C19H17N3O3
Molecular Weight	335.36 g/mol
Synonyms	L-Phenylalanine 7-amido-4-methylcoumarin; Phe-AMC; L-Phe-AMC; N-Succinyl-L-phenylalanine 7-amido-4-methylcoumarin; Suc-Phe-AMC; Protease Substrate AMC; 7-Amino-4-methylcoumarin conjugate of L-Phenylalanine; Fluorogenic protease substrate
EINECS	Contact for details

Quality Control

Every batch of L-Phe-7-Amino-4-Methylcoumarin is manufactured and tested under strict quality management protocols to ensure high purity and consistent performance. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.