Description

Guanosine 5'-o-(2-Thiodiphosphate) Trilithium Salt is a high-purity, modified nucleotide analog where a sulfur atom replaces one of the non-bridging oxygen atoms in the diphosphate moiety. This compound is a critical biochemical tool for studying enzyme mechanisms, particularly GTPases and kinases, as it acts as a hydrolysis-resistant or slow-hydrolyzing GTP analog. It is essential for researchers and manufacturers in biochemical research, enzymology, and pharmaceutical development who require precise probes to investigate nucleotide-dependent signaling pathways and protein function.

Application

• Enzymology Research: Used as a non-hydrolyzable or slowly hydrolyzable GTP analog to study the mechanisms of GTP-binding proteins (G-proteins), GTPases, and other nucleotide-dependent enzymes.
• Signal Transduction Studies: Essential for probing and modulating cellular signaling pathways that rely on GTP/GDP cycling, such as those involving Ras, Rho, and Rab family proteins.
• Kinase Assay Development: Serves as a substrate or inhibitor analog in assays for protein kinases and other phosphotransferase enzymes to understand substrate specificity and inhibition.
• Structural Biology: Utilized in X-ray crystallography and NMR studies to capture and determine the structures of enzyme-substrate or enzyme-inhibitor complexes.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify novel modulators (agonists or antagonists) of GTPase activity for therapeutic targeting.
• Biochemical Reagent Production: A key raw material for diagnostic kit manufacturers and companies producing specialized assay kits for research and clinical diagnostics.

Basic Information

Product Name	Guanosine 5'-o-(2-Thiodiphosphate) Trilithium Salt
CAS No.	97952-36-8
Molecular Formula	C10H12Li3N5O12P2S
Molecular Weight	519.07 g/mol (anhydrous basis)
Synonyms	Guanosine 5'-[β-thio]diphosphate trilithium salt; GDPβS trilithium salt; Guanosine-5'-O-[2-thiodiphosphate] trilithium salt; 5'-Guanylyl (β-thio)diphosphorate trilithium salt; Gpp(S)p trilithium salt; 2'-Deoxyguanosine-5'-O-[1-thiodiphosphate] trilithium salt (related analog); Non-hydrolyzable GTP analog; GTPγS analog
EINECS	Contact for details

Quality Control

Our Guanosine 5'-o-(2-Thiodiphosphate) Trilithium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high chemical purity suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to rigorous analytical standards, employing techniques such as HPLC, NMR, and mass spectrometry for verification. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). For long-term storage, keep under an inert atmosphere or in a vacuum desiccator to maintain stability and purity. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Lithium Content (ICP)	Conforms to theoretical
Solubility	Soluble in water, buffer solutions
Biological Activity Test	Inhibits GTP hydrolysis in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.