Description

5-(n-Biotinyl-3-Aminoallyl)Uridine 5'-Monophosphate is a biotin-labeled nucleotide derivative, a critical reagent for the sensitive detection and analysis of nucleic acids. Its core value lies in enabling high-affinity, non-radioactive labeling for applications in molecular biology and diagnostics. This compound is essential for researchers and manufacturers in the fields of next-generation sequencing (NGS) probe development, microarray fabrication, and in situ hybridization, where precise and stable biotin conjugation is required.

Application

• Nucleic Acid Labeling & Detection: Primary reagent for incorporating biotin tags into DNA or RNA probes for use in blotting, hybridization, and pull-down assays.
• Next-Generation Sequencing (NGS): Used in the synthesis of biotinylated primers or probes for target enrichment, library preparation, and sequencing-by-capture technologies.
• Microarray & Biochip Fabrication: Enables the creation of biotin-functionalized nucleic acid spots for high-density arrays used in genomics and diagnostics.
• In Situ Hybridization (ISH/FISH): Serves as a key component for generating non-radioactive, enzyme-linked probes for cellular and tissue-based localization of genetic sequences.
• Affinity Purification & Pull-Down Assays: Facilitates the isolation of specific DNA/RNA sequences or protein complexes via streptavidin-biotin interaction.
• Diagnostic Kit Manufacturing: A vital raw material in the production of kits for infectious disease testing, genetic screening, and companion diagnostics.

Basic Information

Product Name	5-(n-Biotinyl-3-Aminoallyl)Uridine 5'-Monophosphate
CAS No.	97950-37-3
Molecular Formula	C24H35N6O12P
Molecular Weight	630.55 g/mol
Synonyms	5-(N-Biotinyl-3-aminoallyl)uridine 5'-monophosphate; Biotin-AA-UTP; 5-(3-Aminoallyl)-N-biotinyluridine 5'-monophosphate; Biotinylated UMP; Biotin-allylamine-dUTP (reduced form); 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[3-[(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)methoxy]prop-2-en-1-yl]pentanamide 5'-(dihydrogen phosphate); Biotin-AA-UMP
EINECS	Contact for details

Quality Control

Our production of 5-(n-Biotinyl-3-Aminoallyl)Uridine 5'-Monophosphate adheres to stringent quality protocols suitable for life science research and in-vitro diagnostic (IVD) applications. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural identity and high purity. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.