Description

n-Ethylmaleimide-S-Glutathione is a specific, covalent adduct formed between the thiol-reactive reagent N-ethylmaleimide (NEM) and the tripeptide glutathione. This compound is of significant value as a biochemical tool and reference standard for studying thiol-mediated biological processes, particularly protein glutathionylation and redox signaling. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and biochemical research who require precise molecular probes to investigate enzyme inhibition, oxidative stress pathways, and protein function.

Application

• Biochemical Research: Used as a stable, characterized adduct to study the kinetics and mechanisms of thiol modification and protein S-glutathionylation.
• Proteomics & Mass Spectrometry: Serves as a critical reference standard and calibrant for the identification and quantification of glutathionylated peptides in complex biological samples.
• Enzyme Inhibition Studies: Acts as a tool compound to investigate enzymes with active-site cysteine residues, such as dehydrogenases and phosphatases, by mimicking a glutathionylated state.
• Pharmaceutical Development: Employed in the screening and validation of drug candidates targeting redox-regulated pathways and diseases related to oxidative stress.
• Quality Control Standard: Utilized as an analytical standard in laboratories to validate assays for detecting and measuring glutathione conjugates.

Basic Information

Product Name	n-Ethylmaleimide-S-Glutathione
CAS No.	97908-43-5
Molecular Formula	C₁₅H₂₁N₃O₈S
Molecular Weight	403.41 g/mol
Synonyms	N-Ethylmaleimide Glutathione Adduct; S-(N-Ethylsuccinimido)glutathione; Glutathione-N-ethylmaleimide Conjugate; NEM-Glutathione; GS-NEM; Glutathione N-Ethylmaleimide; NEM-GSH; L-γ-Glutamyl-L-cysteinylglycine N-ethylmaleimide
EINECS	Contact for details

Quality Control

Our n-Ethylmaleimide-S-Glutathione is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via mass spectrometry, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.