Description

3-(10-Pyrenedecanoyl)Cholesterol is a synthetic, fluorescently labeled cholesterol derivative where a pyrene moiety is attached via a ten-carbon linker. This compound matters for advanced research as it serves as a crucial molecular probe for studying lipid dynamics, membrane fluidity, and intracellular cholesterol trafficking. Researchers in biophysics, cell biology, and pharmaceutical development need it to visualize and quantify processes within biological membranes using fluorescence spectroscopy and microscopy techniques.

Application

• Fluorescence Resonance Energy Transfer (FRET) Studies: Acts as a donor or acceptor fluorophore to investigate lipid-protein interactions and membrane domain organization.
• Membrane Fluidity and Microviscosity Analysis: Used to probe the physical properties of lipid bilayers and model membranes through pyrene excimer formation.
• Intracellular Cholesterol Trafficking Visualization: Tracks the movement and localization of cholesterol analogs in live cells using fluorescence microscopy.
• Lipoprotein and Lipid Raft Research: Incorporated into lipoproteins or membrane rafts to study their structure, stability, and function.
• Development of Diagnostic Assays: Serves as a key component in developing fluorescence-based assays for cholesterol-related metabolic disorders.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify compounds that modulate cholesterol metabolism or membrane properties.

Basic Information

Product Name	3-(10-Pyrenedecanoyl)Cholesterol
CAS No.	97850-83-4
Molecular Formula	C48H64O2
Molecular Weight	673.02 g/mol
Synonyms	Cholesteryl 10-(1-Pyrenyl)decanoate; 10-(1-Pyrenyl)decanoyl Cholesterol; 3β-[[10-(1-Pyrenyl)decanoyl]oxy]cholest-5-ene; N-((10-Pyren-1-yl-decanoyl)-cholesterol); Pyrene-labeled Cholesterol; Fluorescent Cholesterol Derivative; Pyrene-C10-Cholesterol; Cholesterol Pyrenedecanoate
EINECS	Contact for details

Quality Control

Our 3-(10-Pyrenedecanoyl)Cholesterol is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC, NMR, and mass spectrometry, to confirm identity and purity. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen). Allow the product to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation. The original container is the primary moisture barrier; do not transfer to other containers unless absolutely necessary.

Specification

Item	Specification
Appearance	Yellow to light brown solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Pyrene Content (UV-Vis)	Conforms to theoretical
Solubility	Soluble in DMSO, DMF, chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.