Description

7-(n-Benzyloxycarbonyl-β-Benzylaspartyl-Prolyl-Leucyl)Amino-4-Methylcoumarin is a specialized synthetic peptide derivative incorporating a fluorogenic coumarin moiety. This compound is primarily valued as a highly sensitive and selective substrate for enzymatic activity studies, particularly in protease research. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require precise tools for enzyme kinetics, inhibitor screening, and assay development.

Application

• Protease & Enzyme Assay Development: Acts as a fluorogenic substrate for the study of aspartyl proteases, including renin and cathepsin D, enabling real-time kinetic analysis.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery for screening libraries of compounds to identify potential protease inhibitors.
• Biochemical Research: A critical tool in academic and industrial labs for investigating enzyme mechanisms, specificity, and catalytic efficiency.
• Diagnostic Reagent Component: Employed in the formulation of sensitive diagnostic kits for detecting specific protease activities linked to disease states.
• Quality Control in Biologics: Supports activity testing and potency assays for biopharmaceutical products involving proteolytic enzymes.

Basic Information

Product Name	7-(n-Benzyloxycarbonyl-β-Benzylaspartyl-Prolyl-Leucyl)Amino-4-Methylcoumarin
CAS No.	97792-42-2
Molecular Formula	C43H50N6O9
Molecular Weight	794.90 g/mol
Synonyms	Z-β-Asp(OBzl)-Pro-Leu-AMC; 7-(N-Cbz-β-Benzyl-Aspartyl-Prolyl-Leucyl)Amino-4-Methylcoumarin; Cbz-β-Benzyl-Asp-Pro-Leu-AMC; Fluorogenic Substrate for Aspartic Proteases; Renin Substrate AMC; Cathepsin D Substrate; Aspartic Protease Fluorogenic Peptide Substrate; AMC-labeled Peptide Substrate
EINECS	Contact for details

Quality Control

Every batch of our 7-(n-Benzyloxycarbonyl-β-Benzylaspartyl-Prolyl-Leucyl)Amino-4-Methylcoumarin is manufactured and tested under a strict quality management system. We ensure compliance with the high-purity standards required for research and diagnostic applications. Comprehensive analytical testing, including HPLC for purity and identity confirmation, is performed. A Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic nature, allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (Mass Spectrometry)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (Karl Fischer)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.