Description

Z-Gly-Gly-Leu-Amc is a fluorogenic peptide substrate designed for the sensitive detection and study of protease enzyme activity. This compound is a critical tool for biochemical research and pharmaceutical development, offering a reliable mechanism to quantify enzymatic cleavage. It is primarily utilized by researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical industries for high-throughput screening and kinetic assays.

Application

• Protease Enzyme Assays: A standard fluorogenic substrate for measuring the activity of various proteases, including aminopeptidases and specific endoproteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential protease inhibitors as therapeutic candidates.
• Biochemical Research: Essential for studying enzyme kinetics, substrate specificity, and catalytic mechanisms in academic and industrial R&D settings.
• Diagnostic Development: Employed in the development of fluorometric diagnostic kits for detecting protease-related disease biomarkers.
• Quality Control in Biologics: Used to monitor and validate the activity of enzyme-based products and biomanufacturing processes.

Basic Information

Product Name	Z-Gly-Gly-Leu-Amc
CAS No.	97792-39-7
Molecular Formula	C₂₃H₃₂N₄O₇
Molecular Weight	476.53 g/mol
Synonyms	Z-Gly-Gly-Leu-AMC; Benzyloxycarbonyl-Gly-Gly-Leu-7-amido-4-methylcoumarin; Cbz-Gly-Gly-Leu-AMC; Z-Gly-Gly-Leu-7-amino-4-methylcoumarin; Substrate for Aminopeptidase; Fluorogenic Peptide Substrate 97792-39-7; N-Carbobenzoxy-glycyl-glycyl-L-leucine 7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Z-Gly-Gly-Leu-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.