Description

D-Val-Leu-Lys-7-Amino-4-Trifluoro-Methylcoumarin is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critically important for researchers and quality control laboratories requiring precise detection and quantification of protease activity, particularly for enzymes like plasmin and trypsin. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, biochemical research, and assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, real-time monitoring of enzyme kinetics for proteases such as plasmin, trypsin, and related serine proteases.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: A key tool for studying enzyme mechanisms, specificity, and inhibition constants (Ki) in academic and industrial research settings.
• Diagnostic Kit Development: Incorporated into in-vitro diagnostic (IVD) kits for the detection of specific protease biomarkers associated with various disease states.
• Quality Control in Biologics: Employed to monitor and validate the activity and purity of enzyme-based biopharmaceutical products during manufacturing.

Basic Information

Product Name	D-Val-Leu-Lys-7-Amino-4-Trifluoro-Methylcoumarin
CAS No.	97055-77-1
Molecular Formula	C31H41F3N6O6
Molecular Weight	650.69 g/mol
Synonyms	D-Val-Leu-Lys-AFC; D-Val-Leu-Lys-AMC (AFC derivative); AFC Substrate D-Val-Leu-Lys; 7-Amino-4-trifluoromethylcoumarin-D-Val-Leu-Lys; Plasmin Substrate (Fluorogenic); Boc-Val-Leu-Lys-AFC; Val-Leu-Lys-AFC (D-isomer); Fluorogenic Plasmin Substrate VLK-AFC
EINECS	Contact for details

Quality Control

Our D-Val-Leu-Lys-7-Amino-4-Trifluoro-Methylcoumarin is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay (Spectrophotometric)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.