Description

Restriction Endonuclease Ssp I is a high-purity, site-specific endonuclease enzyme used for precise DNA cleavage in molecular biology applications. This product is essential for researchers and industrial biotechnologists requiring reliable and consistent performance in genetic engineering, cloning, and genomic analysis workflows. It is a critical reagent for pharmaceutical R&D, academic research, and diagnostic kit manufacturing where accurate DNA manipulation is paramount.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into vectors.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used in genetic fingerprinting and mutation detection.
• Genomic DNA Mapping: Facilitates the construction of physical maps of genomes.
• Diagnostic Assay Development: A key component in the manufacture of kits for genetic testing and pathogen detection.
• Site-Directed Mutagenesis: Used in protein engineering and functional genomics studies.
• DNA Sequencing Library Preparation: Aids in the fragmentation and preparation of DNA for next-generation sequencing.

Basic Information

Product Name	Restriction Endonuclease Ssp I
CAS No.	97002-84-1
Molecular Formula	Protein
Molecular Weight	Contact for details
Synonyms	SspI; Endodeoxyribonuclease SspI; Ssp I Restriction Enzyme; Type II Site-Specific Deoxyribonuclease SspI; EC 3.1.21.4; Restriction Enzyme Ssp I
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Ssp I is manufactured under stringent quality systems. Each lot undergoes comprehensive testing for activity, specificity, and purity, ensuring it is free from contaminating nucleases and phosphatases. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our rigorous specifications for use in sensitive research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The supplied storage buffer is optimized to maintain enzymatic activity.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves AATATT sequence specifically
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95% single band
Contaminating Activities	Exonuclease: Not detected Endonuclease: Not detected Phosphatase: Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.